62305

Lipase from Rhizopus oryzae

powder (fine), ~10 U/mg

• Synonim(y): Lipase from Rhizopus arrhizus, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numer CAS: 9001-62-1
• Numer EC enzymu: 3.1.1.3 (BRENDA, IUBMB)
• Numer WE: 232-619-9
• Numer MDL: MFCD00131509
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• Formularz: powder (fine)
• Poziom jakości: 100
• aktywność właściwa: ~10 U/mg
• masa cząsteczkowa: M_r ~43000
• temp. przechowywania: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Klucz InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Opis

Opis ogólny

Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence.[1]

Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.[2]

Zastosowanie

Lipase from Rhizopus oryzae has been used:

• to test its effect on 1,2-diolein synthesis and triolein ethanolysis[3]
• for immobilization on graphene oxide support for biocatalysis studies[4]
• to digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae[5]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Działania biochem./fizjol.

Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.[2]

Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis.[6] ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality.[1]

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Definicja jednostki

1 U corresponds to the amount of enzyme which liberates 1 μmol of butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010 as substrate) 5000 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40°C

Inne uwagi

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Catalyst for the interesterification of oils and fats[7]; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B[8]; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis[9]

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)