Solutions of Fast Green FCF are used much like solutions of Light Green SF Yellowish. The advantage of using Fast Green FCF is generally that the stain is more intense. The working solution is typically prepared fresh each day or used for a short period of time.
F7258
Fast Green FCF
certified by the BSC
Synonim(y):
Food green 3
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Wybierz wielkość
25 G
1060,00 zł
1060,00 zł
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Informacje o tej pozycji
Wzór liniowy:
C37H34N2O10S3Na2
Numer CAS:
Masa cząsteczkowa:
808.85
EC Number:
219-091-5
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5718212
Colour Index Number:
42053
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certified by the BSC
Quality Level
form
powder
composition
dye content, ≥90%
mp
290 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
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Ta pozycja | |||
|---|---|---|---|
| grade certified by the Biological Stain Commission | grade - | grade certified by the Biological Stain Commission | grade - |
| solubility H2O: 1 mg/mL | solubility H2O: 1 mg/mL, clear | solubility H2O: 1 mg/mL, blue | solubility H2O: 1 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. room temp |
| composition dye content, ≥90% | composition Dye content, ≥85% | composition Dye content, ≥90% | composition Dye content, ≥85% |
Application
Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Fast Green FCF has been used:
- along with safranin for the staining of nodule sections
- in the collagen deposit staining procedure[1]
- for the staining of protein and starch
- as a dye to detect and inject DNA solution in chicken embryo
Ta strona może zawierać tekst przetłumaczony maszynowo.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2 - STOT SE 3
target_organs
Respiratory system
Klasa składowania
11 - Combustible Solids
wgk
WGK 2
flash_point_f
352.4 °F - closed cup
flash_point_c
178 °C - closed cup
ppe
Eyeshields, Gloves
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Fernandez-Luque?o F et al.
Acta Physiologiae Plantarum / Polish Academy of Sciences, Committee of Plant Physiology Genetics and Breeding, 30, 545-552 (2008)
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| F7258-25G | 04061833619421 |
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What is the proper method to prepare Fast Green FCF for use as a counterstain?
1 answer-
Helpful?
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Looking for a green dye to add to electrolyzed water. Something biodegradable that will disappear fast on skin.
1 answer-
It is advised to source a FD&C (Food, Drug & Cosmetic) grade dye for use on human skin. Assuming there is suitable electrolyzed water, green food coloring should suffice for the intended purpose. The duration for which the dye remains on the skin would depend on factors such as the pH of the water used and the specific characteristics of the dye, which the customer would need to determine.
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