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69966

MRS Broth

Name: MRS Broth
Brand: SIAL
Price: 960 PLN

NutriSelect^® Basic, powder, for Lactobacillus spp., for Bifidobacterium spp., pack of 500 g

Synonim(y):

Lactobacillus Broth acc. to De Man, Rogosa and Sharpe

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Gabaryty przesyłki	SKU	Dostępność	Cena netto
500 g		Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności	960,00 zł

Informacje o tej pozycji

NACRES:

NA.74

UNSPSC Code:

41171607

Manufacturer/tradename:

NutriSelect^® Basic

Ph:

6.2±0.2

Form:

powder

Suitability:

Bifidobacterium spp., Lactobacillus spp.

Application(s):

agriculture
bioburden testing
environmental
food and beverages
microbiology

960,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

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Właściwości

sterility

non-sterile

Quality Segment

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 2 g/L , glucose, 20 g/L , magnesium sulfate monohydrate, 0.2 g/L , manganous sulfate tetrahydrate, 0.05 g/L , meat extract, 8 g/L , peptone, 10 g/L , sodium acetate, 5 g/L , triammonium citrate, 2 g/L , yeast extract, 4 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect^® Basic

technique(s)

microbiological culture: suitable

pH

6.2±0.2

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

Bifidobacterium spp., Lactobacillus spp.

Opis

General description

MRS Broth is used for the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe. The medium can be used for confirmatory tests of colonies isolated from MRS Agar. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium, and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species, as well as other secondary bacteria, may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide, and polymyxin. These substances can be used at varying concentrations and combinations, but inevitably a compromise must be reached between selectivity and recovery rate of the organism.

Application

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material

Preparation Note

Dissolve 51.25 g in 1 litre distilled water and add 1 ml Tween^® 80 (Cat. No. P8074). Boil to dissolve the medium completely. Fill into containers and sterilize by autoclaving at 121°C for 15 minutes. lncubate the culture up to 3 days at 35°C or up to 5 days at 30°C. If possible, incubate the culture in a CO2 enriched atmosphere in an anaerobic jar.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.10661 - MRS broth

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

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Ta pozycja	70122	69965	38944
Millipore 69966 MRS Broth	Millipore 70122 Nutrient Broth	Millipore 69965 Mossel Broth	Millipore 38944 MRS Broth modified, Vegitone
form powder	form powder	form powder	form powder
technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable
suitability Bifidobacterium spp., Lactobacillus spp.	suitability Erysipelothrix spp., bacteria, nonselective for Brucella spp., nonselective for Escherichia coli, nonselective for Listeria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms	suitability selective for Citrobacter spp., selective for Enterococcus spp., selective for Escherichia coli, selective for Klebsiella spp., selective for Proteus spp., selective for Salmonella spp., selective for Shigella spp., selective for Yersinia spp., selective for coliforms, selective for enterobacteriaceae	suitability selective by low pH for Bifidobacterium spp., selective by low pH for Lactobacillus spp.
manufacturer/tradename NutriSelect^® Basic	manufacturer/tradename NutriSelect^® Plus	manufacturer/tradename NutriSelect^® Basic	manufacturer/tradename NutriSelect^® Plus
application(s) agriculture bioburden testing environmental food and beverages microbiology	application(s) agriculture bioburden testing environmental food and beverages life science and biopharma microbiology	application(s) agriculture bioburden testing environmental food and beverages water monitoring, microbiology	application(s) agriculture bioburden testing environmental food and beverages, microbiology
composition dipotassium hydrogen phosphate, 2 g/L , magnesium sulfate monohydrate, 0.2 g/L , meat extract, 8 g/L , sodium acetate, 5 g/L , yeast extract, 4 g/L , glucose, 20 g/L , peptone, 10 g/L , manganous sulfate tetrahydrate, 0.05 g/L , triammonium citrate, 2 g/L	composition D(+)-glucose, 1 g/L , peptone, 15 g/L , sodium chloride, 6 g/L , yeast extract, 3 g/L	composition brilliant green, 0.015 g/L , disodium hydrogen phosphate, 6.45 g/L , glucose, 5 g/L , ox-bile, 20 g/L , peptone, 10 g/L , potassium dihydrogen phosphate, 2 g/L	composition ammonium citrate, 2 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20 g/L , dipotassium phosphate, 2 g/L , magnesium sulfate, 0.10 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3 g/L , proteose peptone (vegetable), 10 g/L , sodium acetate, 5 g/L , yeast extract, 5 g/L

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Klasa składowania

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Powiązane treści

Data Sheet - 69966

Dehydrated Culture Media Brochure

L.C. Laleye et al.

Journal of Food Protection, 50, 1009-1009 (1987)

Cell-wall-associated proteinase of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397: differential extraction, purification and properties of the enzyme.

P Laloi et al.

Applied microbiology and biotechnology, 36(2), 196-204 (1991-11-01)

Whole cells of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 were able to hydrolyse alpha- and beta-caseins. Irrespective of the growth medium used, milk or De Man-Rogosa-Sharpe (MRS) broth, identical patterns of alpha- and beta-casein hydrolytic products, respectively, were visualized by

J.D. DeMan et al.

The Journal of Applied Bacteriology, 23, 130-130 (1960)

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Numer pozycji handlu globalnego

SKU	NUMER GTIN
69966-500G	04061832795140

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