50484

Methylene Blue solution

suitable for microbiology

• Synonim(y): Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Wzór empiryczny (zapis Hilla): C₁₆H₁₈ClN₃S
• Numer CAS: 61-73-4
• Masa cząsteczkowa: 319.85
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 329757603
• MDL number: MFCD00012111
• Beilstein/REAXYS Number: 3641570

Właściwości

• shelf life: limited shelf life, expiry date on the label
• concentration: 0.5% in H₂O (0.5 g/100 mL)
• technique(s): microbe id | staining: suitable
• application(s): microbiology
• suitability: Bacillus spp., Corynebacterium spp., bacteria
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Opis

General description

Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Other Notes

reagent contains:
methylene blue 0.5 g
distilled water 100 ml

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves