03978

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

• Synonim(y): Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Wzór empiryczny (zapis Hilla): C₁₆H₁₈ClN₃S
• Numer CAS: 61-73-4
• Masa cząsteczkowa: 319.85
• Beilstein: 3641570
• Numer MDL: MFCD00012111
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 329747853
• NACRES: NA.47

Właściwości

• klasa czystości: for microscopy
• Postać: liquid
• jakość: concentrated, aqueous solution
• okres trwałości: ~3 yr
• stężenie: 1.5% (1.5 g/ 100 mL)
• gęstość: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: room temp
• ciąg SMILES: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• Klucz InChI: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Opis

Opis ogólny

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Zastosowanie

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter