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RPOLT3-RO
Roche
T3 RNA Polymerase
from Escherichia coli HB101
Synonim(y):
mRNA, polymerase
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About This Item
Polecane produkty
pochodzenie biologiczne
Escherichia coli (HB101)
Poziom jakości
Próba
100% (SDS-PAGE)
Formularz
solution
aktywność właściwa
≥20 U/μL
masa cząsteczkowa
100 kDa (single polypeptide chain)
opakowanie
pkg of 1,000 U (11031163001)
pkg of 5,000 U (11031171001)
producent / nazwa handlowa
Roche
stężenie
<0.1 % (w/w)
metody
DNA sequencing: suitable
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
kolor
colorless
pH
7.9 (39 °F)
8.0 (68 °F)
rozpuszczalność
water: miscible
przydatność
suitable for molecular biology
numer dostępu NCBI
numer dostępu UniProt
Zastosowanie
genomic analysis
life science and biopharma
obecność zanieczyszczeń
Nicking activity 150 units, none detected
RNases 150 units
endonucleases 150 units, none detected
temp. przechowywania
−20°C
informacje o genach
Escherichia coli ... rpoB(948488)
Powiązane kategorie
Opis ogólny
With this enzyme, homogeneously labeled single-stranded RNA can be generated. Transcripts can be nonradioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides. T3 RNA polymerase is a DNA-dependent RNA polymerase that is highly specific for its promoter. Only DNA downstream from the T3 promoter will be transcribed.
Specyficzność
Promotor specifity
T3 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage T3 DNA or DNA cloned downstream of a T3 promoter.
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or heat to 65 °C.
T3 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage T3 DNA or DNA cloned downstream of a T3 promoter.
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or heat to 65 °C.
Zastosowanie
T3 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T3 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:
- RNA or DNA blotting techniques
- In situ hybridization
- RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
- Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
Opakowanie
1 kit containing 2 components
Jakość
Absence of endonucleases, nicking activity, and RNases tested according to the current Quality Control procedures. Function tested with the SP6/T7 Transcription Kit.
Charakterystyka techniczna
Volume Activity: =20U/μl
Synthesis of hybridization probes: T3 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels: Transcripts can be nonradioactively labeled with biotin-16-UTP, DIG-11-UTP, or fluorescein-12-UTP. They may also be radioactively labeled to high specific activity with [a-32P]- or [a-35S]-labeled nucleotides.
Note: Roche has 10x concentrated RNA Labeling Mixes that are specially designed for DIG-, biotin-, and fluorescein-labeling. These mixes work well with T3 RNA Polymerase.
Synthesis of hybridization probes: T3 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels: Transcripts can be nonradioactively labeled with biotin-16-UTP, DIG-11-UTP, or fluorescein-12-UTP. They may also be radioactively labeled to high specific activity with [a-32P]- or [a-35S]-labeled nucleotides.
Note: Roche has 10x concentrated RNA Labeling Mixes that are specially designed for DIG-, biotin-, and fluorescein-labeling. These mixes work well with T3 RNA Polymerase.
Definicja jednostki
One unit is the enzyme activity which incorporates 1 nMol CMP in acid-precipitable RNA products within 60 minutes at +37 °C.
Volume Activity: ≥20 U/μl
Volume Activity: ≥20 U/μl
Uwaga dotycząca przygotowania
Activator: BSA/spermine
Przechowywanie i stabilność
Keep container tightly closed in a dry and well-ventilated place.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Tylko elementy zestawu
Numer produktu
Opis
- T3 RNA Polymerase, in buffer, pH 7.9 ≥20 U/μl
- Transcription Buffer 10x concentrated
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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