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Merck

11363743001

Roche

Nutridoma-CS

suitable for hybridoma, solution, pkg of 10 mL (50x)

Synonim(y):

serum replacements

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About This Item

Kod UNSPSC:
12352207

sterylność

sterile; 0.2 μm filtered

Poziom jakości

Postać

solution

opakowanie

pkg of 10 mL (50x)

producent / nazwa handlowa

Roche

stężenie

50 ×

metody

cell culture | hybridoma: suitable

zanieczyszczenia

mycoplasma, tested
100 EU/mL endotoxin (LAL test)

rozpuszczalność

water: miscible

temp. przechowywania

−20°C

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Opis ogólny

Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.

Zastosowanie

Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.
Nutridoma-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.

Definicja jednostki

Endotoxin (LAL): 100 EU/ml, mycoplasma tested

Postać fizyczna

Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.

Uwaga dotycząca przygotowania

Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Informacje prawne

Nutridoma is a trademark of Roche

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash


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