C2639

Cell Freezing Medium-Serum-free 1×

Name: Cell Freezing Medium-Serum-free 1×
Brand: SIGMA
Price: 1020 PLN

sterile-filtered, suitable for cell culture

Synonim(y):

Cryopreservation Medium, Serum-Free Freezing Medium

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50 ML

1020,00 zł

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Informacje o tej pozycji

NACRES:

NA.75

UNSPSC Code:

12352207

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Pozwól nam pomóc

sterility

sterile-filtered

Quality Level

100

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

Powiązane kategorie

Cell Culture & Analysis

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Ta pozycja	C9249	C6295	C2999
Sigma-Aldrich C2639 Cell Freezing Medium-Serum-free 1×	Sigma-Aldrich C9249 CryoSOfree^™ DMSO-free Cryopreservation Medium	Sigma-Aldrich C6295 Cell Freezing Medium-DMSO Serum free 1x	Sigma-Aldrich C2999 CryoStor^® cell cryopreservation media
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable	technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable	technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable	technique(s) cell culture \| mammalian: suitable, cryopreservation: suitable
sterility sterile-filtered	sterility -	sterility sterile-filtered	sterility sterile-filtered
shipped in ambient	shipped in wet ice	shipped in dry ice	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 100

Application

Sigma′s Cell Freezing Medium, Serum-free is a cryopreservation medium which does not contain DMSO or serum.

Cell Freezing Medium-Serum-free 1× has been used as a cryopreservation medium for somatic cell sampling and recovery (SCSR) exfoliated cells cultured in growth media and dihydrofolate reductase (DHFR)-deficient Chinese hamster ovary (CHO) cells grown in serum-free growth medium.[1]

Other Notes

Ready-to-use formula that does not contain dimethyl sulfoxide or serum.

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Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Overproduction of recombinant human hepatocyte growth factor in Chinese hamster ovary cells.

Jeong Soo Park et al.

Protein expression and purification, 70(2), 231-235 (2009-10-13)

Hepatocyte growth factor (HGF) is a potent multi-functional protein that affects morphogenesis, cell migration, organ regeneration, and tumor invasion in various tissues, and has thus been considered to have potential as a therapeutic target in various diseases. In our current

Identification of a native novel oncolytic immunoglobulin on exfoliated colon epithelial cells: A bispecific heterodimeric chimera of IgA/IgG

Albaugh G P, et al.

Open Medicine : A Peer-Reviewed, Independent, Open-Access Journal, 10(6), 126-150 (2020)

Numer pozycji handlu globalnego

SKU	NUMER GTIN
C2639-50ML	04061833484388

Questions

a year ago

Hi there, can i use this C2639 Cell Freezing Medium-Serum-free 1× for freezing HEK293 and MCF7 cells? In addition, can i use it to freeze hESC and hiPSC?

1 answer
1. Technical Support
  
  a year ago
  
  This Cell Freezing Medium-Serum-free 1× does not contain dimethyl sulfoxide or serum and may not be suitable for all cell types. It is recommended to follow manufacturers' guidelines or literature for cell freezing. For example:
  - Human kidney (embryonic) 293 cells, Cat. #85120602-1VL: 5% dimethyl sulfoxide and 95% fetal calf serum.
  - MCF7 cells: 95% complete growth medium and 5% dimethyl sulfoxide.
  - Simplicon™ Human iPS cells: pluripotent media with 10% dimethyl sulfoxide.
  - Human embryonic stem cells: base medium with fetalbovine serum and 10% dimethyl sulfoxide.
  For cryopreservation of human embryonic stem cells or induced pluripotent stem cells in serum-free conditions, PluriSTEM™ Freeze Medium (SCM134) is recommended.
  
  Helpful?

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Cell Freezing Medium-Serum-free 1×