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11363514910
Roche
DIG Luminescent Detection Kit
sufficient for 50 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization
Synonim(y):
luminescent detection kit
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50 TESTS
4060,00 zł
4060,00 zł
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50 TESTS
4060,00 zł
About This Item
Kod UNSPSC:
41116134
4060,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Polecane produkty
Próba
>98% (NMR)
Poziom jakości
zastosowanie
sufficient for 50 blots (10 cm x 10 cm each)
opakowanie
kit of 1 (5 components)
producent / nazwa handlowa
Roche
charakterystyka ekologicznej alternatywy
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
metody
hybridization: suitable
kategoria ekologicznej alternatywy
, Aligned
temp. przechowywania
−20°C
Opis ogólny
DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. The nonradioactive DIG system uses digoxigenin, a steroid hapten, coupled to deoxyuridine triphosphate (dUTP), UTP or didUTP to label DNA, RNA or oligonucleotides for hybridization and subsequent luminescent detection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Zastosowanie
DIG Luminescent Detection Kit has been used for highly sensitive and specific detection of DIG-labeled nucleic acids in:
- critical commercial assays[1]
- Southern hybridization[2][3]
- northern analysis[4]
- plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD[2][4]. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.
Opakowanie
1 kit containing 5 components.
Charakterystyka techniczna
Sensitivity: 0.03 pg of homologous DNA or 0.1 pg of homologous RNA is detected in a Southern or dot blot after <30-min exposure time. Single-copy genes are detected in 0.3 μg mammalian genomic DNA.
Uwaga dotycząca przygotowania
Working concentration: 0.5mg/ml for flow cytometry
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
Note: Store protected from light.
- Storage conditions (working solution): Antibody conjugate (vial 3): once opened, should be stored at 2 to 8°C
- Blocking Reagent (bottle 4): in solution at 2 to 8°C
- CSPD (vial 5): at 2 to 8°C when frequently used. Repeated freeze/thaw cycles should be avoided.
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
- Immunological detection 1.5 hours
- Signal detection 5 to 30 minute
Note: Store protected from light.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Tylko elementy zestawu
Numer produktu
Opis
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer
- Anti-digoxigenin-AP antibody, Fab fragments 750 U/ml
- Blocking Reagent
- CSPD 11.6 mg/ml
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
does not flashNot applicable
Temperatura zapłonu (°C)
does not flashNot applicable
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Produkty
Metody znakowania digoksygeniną (DIG) i zestawy do sond DNA i RNA DIG, znakowanie DNA z losowym primerem, znakowanie nickiem translacyjnym, znakowanie końcowe oligonukleotydów 5' i 3'.
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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