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11093657910
Roche
DIG DNA Labeling and Detection Kit
Synonim(y):
dna labeling and detection kit, dig
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wybierz wielkość
25 REACTIONS
4830,00 zł
4830,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Wybierz wielkość
Zmień widok
25 REACTIONS
4830,00 zł
About This Item
Kod UNSPSC:
41105500
4830,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Polecane produkty
zastosowanie
sufficient for 25 labeling reactions
sufficient for 50 blots
Poziom jakości
producent / nazwa handlowa
Roche
charakterystyka ekologicznej alternatywy
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
kategoria ekologicznej alternatywy
, Aligned
temp. przechowywania
−20°C
Opis ogólny
DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers.
Contents:
Contents:
- Unlabeled Control DNA 1, 100 μg/ml
- Unlabeled Control DNA 2, 200 μg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA, 5.2 μg/ml
- 10x Hexanucleotide Mix
- 10x dNTP Labeling Mixture
- Klenow Enzyme, Labeling grade, 2 U/μl
- Anti-digoxigenin-AP-conjugate, 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Specyficzność
Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.
Zastosowanie
DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques:
DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light blue precipitate.
DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light blue precipitate.
Opakowanie
1 kit containing 10 components.
Uwaga dotycząca przygotowania
Working concentration: Dilution of Antibody 1:5,000
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Tylko elementy zestawu
Numer produktu
Opis
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 200 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
- Anti-digoxigenin-AP-conjugate antibody 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
Zobacz wszystko (10)
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
does not flashNot applicable
Temperatura zapłonu (°C)
does not flashNot applicable
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Produkty
Metody znakowania digoksygeniną (DIG) i zestawy do sond DNA i RNA DIG, znakowanie DNA z losowym primerem, znakowanie nickiem translacyjnym, znakowanie końcowe oligonukleotydów 5' i 3'.
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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