MABT205
Anti-α-Tubulin Antibody, clone DM1A
clone DM1A, from mouse
Synonim(y):
Tubulin alpha-1 chain
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
DM1A, monoclonal
reaktywność gatunkowa
mouse, human
reaktywność gatunkowa (przewidywana na podstawie homologii)
chicken (based on 100% sequence homology)
metody
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... TUBA1A(7846)
Opis ogólny
Tubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes.
Immunogen
Native chicken brain microtubules.
Zastosowanie
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells.
Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
Immunohistochemistry Analysis: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-α-Tubulin Antibody, clone DM1A is validated for use in Western Blotting, ICC, IHC for the detection of α-Tubulin.
Jakość
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.
Opis wartości docelowych
~60 kDa observed. Uniprot describes a molecular weight at ~46 kDA, but can be higher due to post-translational modification. This protein has been observed at ~55 kDa in some cell lysates (Saugstad, J. A., et al. (2002). J Neurochem. 80(6):980-988.).
Powiązanie
Replaces: 04-1117
Postać fizyczna
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
HeLa cell lysate.
HeLa cell lysate.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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