About This Item
Kod UNSPSC:
12352203
NACRES:
NA.43
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
CL5, monoclonal
masa cząsteczkowa
calculated mol wt 65.34 kDa
observed mol wt ~91 kDa
reaktywność gatunkowa
human
opakowanie
antibody small pack of 100 μg
metody
western blot: suitable
izotyp
IgG1
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... CYBB(1536)
Opis ogólny
Cytochrome b-245 heavy chain (UniProt: P04839; also known as CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome) is encoded by the CYBB (also known as NOX2) gene (Gene ID: 1536) in Human. Gp91phox is a multi-pass membrane glycoprotein of the NADPH oxidase family that contains three extracellular domains, six transmembrane domains, and four cytoplasmic domains. It contains four iron binding sites and has a ferric oxidoreductase domain that is localized in the amino acids 54-286. It is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide and plays an important role in the host defense function of phagocytic cells. It also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It can undergo polyubiquitination at lysine 48 by RNF145 that triggers its endoplasmic reticulum-associated degradation. Clone CL5 recognizes 13 amino acid sequence from the N-terminal region and displays reactivity with native and recombinant NOX2. Mutations in CYBB gene are known to cause granulomatous disease that is characterized by the inability of neutrophils and phagocytes to kill ingested leading to life-threatening bacterial and fungal infections. (Ref.: (Ref.: Burritt, JB., et al. (2003). J. Immunol. 170 (12); 6082-6089).
Specyficzność
Clone CL5 is a mouse monoclonal antibody that detects human gp91phox/NOX2. It targets an epitope with in 13 amino acids from the N-terminal, third extracellular domain.
Immunogen
Partially purified human neutrophil flavocytochrome b588.
Zastosowanie
Quality Control Testing
Evaluated by Western Blotting in Human PMN cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected gp91phox (NOX2) in Human PMN cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected gp91phox (NOX2) in Western Blotting applications (Baniulis, D., et. al. (2005). Eur J Haematol. 74(4):337-47; Baniulis, D., et. al. (2005). Biochim Biophys Acta. 1752(2):186-96).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human PMN cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected gp91phox (NOX2) in Human PMN cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected gp91phox (NOX2) in Western Blotting applications (Baniulis, D., et. al. (2005). Eur J Haematol. 74(4):337-47; Baniulis, D., et. al. (2005). Biochim Biophys Acta. 1752(2):186-96).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-gp91phox (NOX2), clone CL5, Cat. No. MABS2194, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain (gp91phox) and is tested for use in Western Blotting.
Postać fizyczna
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at +2°C to +8°C from date of receipt.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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