MABS1931

Anti-CD368/CLEC4D Antibody, clone WEN42

• Synonim(y): C-type lectin domain family 4 member D (UniProt: Q69FH1, also known as C-type lectin superfamily member 8
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: WEN42, monoclonal
• masa cząsteczkowa: observed mol wt ~55 kDa
• oczyszczone przez: using protein G
• reaktywność gatunkowa: rat
• opakowanie: antibody small pack of 100
• metody: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG2a
• sekwencja epitopowa: Extracellular domain
• numer dostępu Protein ID: NP_001003707
• numer dostępu UniProt: Q69FH1
• temp. przechowywania: 2-8°C
• informacje o genach: rat ... Clec4d(362432)

Opis

Specyficzność

Clone WEN42 is a mouse monoclonal antibody that detects C-type lectin domain family 4 member D (CD368/CLEC4D). It targets an epitope within the extracellular domain.

Immunogen

BWZ.36 cells expressing chimeric CD368/CLEC4D

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting with recombinant Rat CD368/CLEC4D protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant fragment of Rat CD368/CLEC4D expressed with Fc region of human IgG1.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD368/CLEC4D in Immunoprecipitation applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).

Immunohistochemistry Applications: A representative lot detected CD368/CLEC4D in Immunohistochemistry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406).

Western Blotting Analysis: A representative lot detected CD368/CLEC4D in Western Blotting applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).

Flow Cytometry Analysis: A representative lot detected CD368/CLEC4D in Flow Cytometry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Opis wartości docelowych

C-type lectin domain family 4 member D (UniProt: Q69FH1; also known as C-type lectin superfamily member 8, CD368) is encoded by the Clec4d (also known as Clecsf8, Mcl) gene (Gene ID: 362432) in rat. CD368 is a membrane surface receptor that is expressed on blood monocytes and neutrophils, as well as on several tissue macrophage populations, including alveolar and peritoneal cavity macrophages. It is a single-pass type II protein that contains a cytoplasmic domain (aa 1-24), a transmembrane domain (aa 25-47), and an extracellular domain (aa 48-218). Its extracellular C-terminal region contains the C-type lectin-like domain (aa 89-206) that contains an evolutionarily conserved folded domain, and a carbohydrate recognition domain containing the Ca2+ binding sites. CD368 acts as a pattern recognition receptor (PRR) of the innate immune system that recognizes damage-associated molecular patterns (DAMPs) of pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. It can form disulfide-linked heterodimer with C-type lectin domain family 4 member E (CLEC4E) and with CLEC6A that forms a PPR against fungal infections. (Ref.: Lobato-Pascual, A., et al. (2013). PLoS ONE 8(2); e57406; Lobato-pascual, A., et al. (2013). Eur. J. Immunol. 43(12); 3167-3174).

Postać fizyczna

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Rekonstytucja

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommended storage: +2°C to +8°C .

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable