MABN1846

Anti-Apelin Receptor/APJ, clone 3C3-7 Antibody

clone 3C3-7, from mouse

• Synonim(y): Apelin receptor, Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3C3-7, monoclonal
• reaktywność gatunkowa: human
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_005152
• numer dostępu UniProt: P35414
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... APLNR(187)

Opis

Opis ogólny

Apelin receptor (UniProt P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID 187) in human. Apelin is an angiotensin converting enzyme 2 (ACE2) substrate that functions as an inotropic and cardiovascular protective peptide. Apelin exerts its physiological effects via its receptor APJ, a seven-transmembrane G protein-coupled receptor that shares significant homology with the Ang II type 1 receptor (AT1R). Apelin/APJ signaling plays an important role in a wide range of pathophysiological effects in the cardiovascular system. Apelin levels are reduced in patients with essential hypertension independent of left ventricular systolic and diastolic dysfunction. Treatment with Apelin has been shown to lower systolic blood pressure levels in hypertensive rats via an NO-dependent mechanism. APJ is a 7-transmembrane (a.a. 27-51, 67-91, 101-125, 145-166, 201-221, 245-271, 285-308) GPCR, having a short N-terminal extracellular portion (a.a. 1-26) and a long C-terminal cytomplasmic tail (a.a. 309-380).

Specyficzność

Clone 3C3-7 detects human Apelin receptor/APJ.

Immunogen

Recombinant full-length human Apelin receptor/APJ.

Zastosowanie

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Apelin receptor/APJ in human prostate cancer tissue, as well as normal cerebral cortex and pancreas tissue sections.

Research Category
Neuroscience

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Apelin Receptor, clone 3C3-7 Antibody is validated for use in Immunohistochemistry (Paraffin), Western Blotting for the detection of APJ.

Jakość

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Apelin receptor/APJ in 10 µg of MCF7 cell lysate.

Opis wartości docelowych

~42 kDa 42.66 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Postać fizyczna

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable