Good at showing doublet bands for the AQP4 isoforms by Western blot, 1:1000 goes a long way. Fair bit of non-specific binding at higher molecular weights which originally messed with the chemiluminescence signal when I was optimising, but this became moot when I stated cutting the membrane to load beta-actin and AQP4 antibodies seperately (at the same time). Would not reccomend trying 1:2000 primary or using NFM to block/dilute, not sure how good it is on PVDF membranes but worked well on my nitorcellulose.
AB3594
Anti-Aquaporin-4 Antibody
CHEMICON®, rabbit polyclonal
Anti-Aquaporin 4 Antibody, CT
Synonim(y):
Mercurial-Insensitive Water Channel
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 50 μL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 2420,00 zł | |
| 200 μL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 3680,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, human, rat
Application:
IHC, WB
Citations:
136
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Anti-Aquaporin 4 Antibody, CT, Chemicon®, from rabbit
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AQP4(361)
General description
38 kDa
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
Immunogen
Epitope: C-terminus
GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).
Application
Detect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.
Immunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion & Transport Channels
Ion & Transport Channels
Biochem/physiol Actions
Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.
Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
Physical form
Format: Purified
ImmunoAffinity Purified: Amber vial of Rb X Aquaporin 4, C-Terminus sold as (part # AB3594-50UL or AB3594-200UL)
Purified rabbit polyclonal, lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide.
50UL vial: Reconstitute with 50µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
200UL vial : Reconstitute with 200µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
50UL vial: Reconstitute with 50µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
200UL vial : Reconstitute with 200µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Preparation Note
Stable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Clear vial of RbX Aquaporin 4, CT Control Antigen(AB3594-50ULb) included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Clear vial of RbX Aquaporin 4, CT Control Antigen(AB3594-50ULb) included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level 200 |
| biological source rabbit | biological source rabbit | biological source mouse | biological source rabbit |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified antibody | antibody form affinity isolated antibody |
| technique(s) immunohistochemistry: suitable, western blot: suitable | technique(s) western blot: suitable | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) flow cytometry: suitable (indirect), immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. −20°C |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in - |
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| AB3594-50UL | 04053252737442 |
| AB3594-200UL | 08436037124464 |
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Good Job (one optimised) - but be careful of non-specific binding
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