551826

QUIN 2, Tetrapotassium Salt

Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield.

• Synonim(y): QUIN 2, Tetrapotassium Salt, 2-{[2- bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8- bis(carboxymethyl)aminoquinoline, 4K, 2-{[2-bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8-bis(carboxymethyl)aminoquinoline, 4K
• Wzór empiryczny (zapis Hilla): C₂₆H₂₃N₃O₁₀ · 4K
• Numer CAS: 73630-23-6
• Masa cząsteczkowa: 693.87
• Numer MDL: MFCD00065487
• Kod UNSPSC: 12352302
• NACRES: NA.25

Właściwości

• Poziom jakości: 100
• Próba: ≥95% (HPLC)
• Postać: solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; protect from light
• kolor: yellow
• rozpuszczalność: water: 10 mg/mL
• fluorescencja: λ_ex 332 nm (high); λ_ex 354 nm (low); λ_em 505 nm (high); λ_em 510 nm (low)
• Warunki transportu: ambient
• temp. przechowywania: −20°C
• InChI: 1S/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4
• Klucz InChI: XAGUNWDMROKIFJ-UHFFFAOYSA-J

Opis

Opis ogólny

Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.

Binding of Ca²⁺ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).

Działania biochem./fizjol.

Cell permeable: no

Primary Target
Calcium Probes

Product does not compete with ATP.

Reversible: no

Ostrzeżenie

Toxicity: Irritant (B)

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.

Inne uwagi

Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.
Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.
Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.
Bryant, D.T. 1985. Biochem. J.226, 613.
Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.
Ashley R.H., et al. 1984. Biochem. J.219, 149.
Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable