1.25062
Fractogel® EMD COO- (M)
MiniChrom Fractogel® COO (M), volume 1 mL, prepacked column
Synonim(y):
MiniChrom Column Fractogel® COO (M), 1 ml
Wybierz wielkość
| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 pkg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 765,00 zł |
Informacje o tej pozycji
765,00 zł
ligand
(Carboxyethyl )
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure, ~300 cm/hr max. flow rate
column I.D. × L
8 mm × 20 mm
volume
1 mL
matrix active group
Methacrylate
mean particle size
40-90 μm
capacity
~100 mL, resin binding capacity
application(s)
mAb
recombinant protein
vaccine development
separation technique
cation exchange
storage temp.
15-25°C
General description
Features and Benefits
- Good resolution between monomers and aggregates
- Efficient aggregate and HCP removal combined with high yield
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Analysis Note
Legal Information
1 of 1
Ta pozycja | |||
|---|---|---|---|
| volume 1 mL | volume 1 mL | volume 5 mL | volume 1 mL |
| ligand (Carboxyethyl ) | ligand iminodiacetic acid | ligand carboxymethyl | ligand - |
| separation technique cation exchange | separation technique affinity | separation technique anion exchange | separation technique - |
| form prepacked column | form prepacked column | form prepacked column | form prepacked column |
| material polypropylene housing | material polypropylene housing | material polypropylene housing | material - |
| capacity ~100 mL, resin binding capacity | capacity ~60 mL, resin binding capacity | capacity ~100 mL, resin binding capacity | capacity - |
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