07-888
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
Upstate®, from rabbit
Synonim(y):
40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline-rich), proline-rich Akt substrate, 40 kDa
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
mouse, human
producent / nazwa handlowa
Upstate®
metody
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
phosphorylation (pThr246)
informacje o genach
human ... AKT1S1(84335)
mouse ... Akt1S1(67605)
Opis ogólny
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
Specyficzność
Recognizes phosphorylated PRAS40 (Thr246)), Mr 40 kDa.
Immunogen
Peptide corresponding to amino acid region encompassing the human phospho-PRAS40 (Thr246).
Zastosowanie
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Jakość
Routinely evaluated by Western Blot on PDGF stimulated NIH3T3 cell lysates.
Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Opis wartości docelowych
Approx. 40 kDa
Postać fizyczna
Format: Purified
Purified rabbit polyclonal IgG in buffer containing PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide and 50% glycerol.
Komentarz do analizy
Control
PDGF stimulated NIH3T3 cells.
PDGF stimulated NIH3T3 cells.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
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Proceedings of the National Academy of Sciences of the USA null
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