The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20°C.
P8170
Protein G−Peroxidase from Streptococcus sp.
recombinant, expressed in unspecified host, lyophilized powder
Synonim(y):
Protein G−HRP from Streptococcus sp.
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Wybierz wielkość
250 μG
712,00 zł
712,00 zł
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Informacje o tej pozycji
Form:
lyophilized powder
Recombinant:
expressed in unspecified host
Przejdź do
Pomoc techniczna
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Pozwól nam pomócQuality Level
recombinant
expressed in unspecified host
conjugate
peroxidase conjugate
form
lyophilized powder
technique(s)
direct ELISA: 1:100,000 using human IgG
storage temp.
−20°C
1 of 4
Ta pozycja | |||
|---|---|---|---|
| recombinant expressed in unspecified host | recombinant expressed in E. coli | recombinant expressed in unspecified host | recombinant - |
| technique(s) direct ELISA: 1:100,000 using human IgG | technique(s) affinity chromatography: suitable | technique(s) direct ELISA: 1:70,000 using human IgG | technique(s) direct ELISA: 1:80,000 using human IgG |
| form lyophilized powder | form aqueous ethanol suspension | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100, 200 |
| conjugate peroxidase conjugate | conjugate - | conjugate biotin conjugate | conjugate peroxidase conjugate |
General description
Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.
Application
Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.
Biochem/physiol Actions
The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.
Preparation Note
Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protokoły
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
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After diluting this, how long does it keep in a fridge? (2-8 degrees Celsius)
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