AB9206

Anti-DARPP-32 Antibody, phosphoThr34

Chemicon^®, from rabbit

• Synonym(s): Anti-DARPP-32, Anti-DARPP32
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, mouse, rat
• Application: WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_032192.2, NM_181505.2
• UniProt accession no.: Q9UD71
• shipped in: dry ice
• target post-translational modification: phosphorylation (pThr34)
• Gene Information: human ... PPP1R1B(84152)

Description

General description

~32 kD

Immunogen

Epitope: phosphoThr34

Synthetic peptide from DARPP-32, phosphoThr34.

Application

Research Category
Neuroscience

Research Sub Category
Reward & Addiction

Signaling Neuroscience

This Anti-DARPP-32 Antibody, phosphoThr34 is validated for use in WB for the detection of DARPP-32.

Western blot: 1:1,000. The antibody recognizes the ~32 kD DARPP-32 protein phosphorylated at Thr34 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes DARPP-32 phosphorylated at threonine 34 .

Physical form

Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg per mL BSA with 50% glycerol.

ImmunoAffinity Purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Cultured primary neurons, rat brain lysate and human breast adenocarcinoma

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2