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Merck

MAK309

Sigma-Aldrich

Bile Acid Assay Kit

sufficient for 100 fluorometric tests

Sinónimos:

Biliary Acid Quantification Kit, Total Bile Acids Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

fluorometric

relevant disease(s)

gastrointestinal diseases; cancer; cardiovascular diseases

storage temp.

−20°C

General description

Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gall stones, colon cancer, etc. Bile acids also exist as sulfate salt forms known as bile acid sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion.

Application

Bile Acid Assay Kit has been used to quantify bile acid levels from plasma.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism in serum, plasma, urine and other biological samples.

Principle

This Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3–hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (γex = 530 nm/γem = 585 nm) is linear to the bile acid concentration in the sample.

Storage Class

10 - Combustible liquids


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Tarek Besheer et al.
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 29(3), 299-307 (2018-05-15)
Chronic hepatitis C (CHC)-related mortality generally results from cirrhosis and subsequent complications. We aimed to investigate the potential role of plasma bile acid levels and ABCB11 1331T > C (V444A, rs2287622) (ATP-binding cassette subfamily B, member 11) gene polymorphism in
Bile acids: chemistry, physiology, and pathophysiology.
Monte M J, et al.
World Journal of Gastroenterology, 15(7), 804-804 (2009)
Hung Phuc Nguyen et al.
Journal of animal science, 98(12) (2020-11-18)
A 16-wk growth trial was conducted to examine the effects of dietary replacement of fish meal by defatted soybean meal (SBM) and fermented soybean meal (FSBM) with taurine supplementation on growth performance, nutrient apparent digestibility coefficient (ADC) and biological parameters
Bile acid transporters and regulatory nuclear receptors in the liver and beyond.
Halilbasic E, et al.
Journal of Hepatology, 58(1), 155-168 (2013)
Ahmed Dawood Mohammed et al.
Nature communications, 13(1), 525-525 (2022-01-28)
Mucosal antibodies maintain gut homeostasis by promoting spatial segregation between host tissues and luminal microbes. Whether and how mucosal antibody responses influence gut health through modulation of microbiota composition is unclear. Here, we use a CD19-/- mouse model of antibody-deficiency

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