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MAK052

Sigma-Aldrich

Ensayos de actividad de la ALT

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

Kit de análisis de actividad de la SGPT, Kit de análisis de actividad de la alanina aminotransferasa, Kit de análisis de actividad de la glutámico-pirúvico transaminasa sérica

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad

uso

sufficient for 100 colorimetric or fluorometric tests

método de detección

colorimetric
fluorometric

enfermedades relevantes

gastrointestinal diseases

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... GPT(2875)
mouse ... GPT(76282)
rat ... GPT(81670)

Descripción general

La alanina aminotransferasa (ALT), también conocida como glutámico-pirúvico transaminasa sérica (SGPT), es una enzima dependiente del fosfato de piridoxal que cataliza la transferencia reversible de un grupo amino desde la alanina hasta el α-cetoglutarato, produciendo piruvato y glutamato. La ALT se encuentra principalmente en el hígado y el suero, pero también está en otros tejidos. La lesión hepatocelular suele provocar un aumento de las concentraciones séricas de ALT, y estas concentraciones pueden utilizarse como marcador de la lesión hepática.

Aplicación

El kit de análisis de actividad de la ALT se ha utilizado para determinar la actividad de la alanina aminotransferasa (ALT) en muestras de suero.[1][2][3]

Características y beneficios

Compatible con sistemas de manipulación de alto rendimiento.

Idoneidad

Idóneo para la determinación de la actividad alanina aminotransferasa (ALT) en muestras biológicas: tejidos, células y suero

Principio

El kit de análisis de actividad de la ALT proporciona un procedimiento simple y directo para medir la actividad de la ALT en una diversidad de muestras biológicas.

La actividad ALT viene determinada por un análisis enzimático acoplado, que da lugar a un producto colorimétrico (570 nm)/fluorométrico (λex = 535/λem = 587 nm), proporcional al piruvato generado. Una unidad de ALT se define como la cantidad de enzima que genera 1,0 μmol de piruvato por minuto a 37 °C.

Producto relacionado

Referencia del producto
Descripción
Precios

92210

Timestrip Plus™ 0 °C

06693

Timestrip Plus™ -20 °C

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H317,H334,H412

Clasificaciones de peligro

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
8L23K07520
8L18K07520
8A24K07520
8K11K07520
8K03K07520

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Questions

1–8 of 8 Questions  

  1. Hi, Does this ALT kit depend on LDH for the ultimate readout? Our goal is to measure liver dysfunction in serum of mice. Would differences in serum LDH influence the results? Thanks Ricky

    1 answer

    1. This kit does not use lactate dehydrogenase (LDH) itself, but the measured results are proportional to the concentration of pyruvate generated. Because LDH (in the presence of NADH) can influence the concentration of pyruvate present, the presence of LDH in a sample may influence the assay results.

      LDH was not assessed in the development of this kit, but the kit has been used to test serum samples directly. It is recommended to perform several sample dilutions to determine the optimal sample concentration and ensure measured values fall within the linear range of the standard curve. Liver cell and liver-derived cell line lysates are more commonly measured, as serum levels of ALT are normally low. Regardless, considering the effect of LDH on pyruvate in the presence of NADH, a variable concentration of LDH and/or NADH may impact the ALT assay results.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. Can I know the specific activity of this ALT mix sample? In unit of U/mg

    1 answer

    1. The ALT Enzyme Mix included in this kit is a lyophilized mixture of enzymes. The components include Pyruvate Oxidase, Cocarboxylase, and Sucrose. The concentration and activity of each enzyme are considered proprietary. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/731/802/mak052bul.pdf

      Helpful?

  5. Is the ALT activity assay kit MAK052 compatible with EDTA plasma? Does EDTA interfere with the activity of the assay?

    1 answer

    1. EDTA has the potential to deactivate the enzyme. ​Therefore, it is recommended not to use samples containing EDTA for performing the AST and ALT assay kits, including the MAK055 kit.

      Helpful?

  6. How does the presence of pyruvate in the Krebs buffer affect the final reaction of the kit, and why is its removal recommended? Additionally, what is the significance of the absence of enzymes in the kit for converting glucose and lactate to pyruvate in relation to the buffer?

    1 answer

    1. The kit's final reaction is dependent on the oxidation of pyruvate, so its presence in the buffer may interfere with the assay. If possible, it is recommended to remove pyruvate from the Krebs buffer. However, the kit does not contain enzymes that can convert glucose to pyruvate or lactate to pyruvate, so the presence of these should not pose an issue in the buffer.

      Helpful?

  7. Can I use the kit for both mouse and human serum?

    1 answer

    1. This kit is suitable for mouse and human serum. As mentioned on the Product Detail Page, this item is suitable for measuring alanine aminotransferase (ALT) activity in biological samples including tissue, cells, and serum.

      Helpful?

  8. Can it be used for measuring on supernatant from liver cells

    1 answer

    1. The kit is optimized for use in determination of alanine aminotransferase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.) This kit has not been tested for use with cell culture supernatant. The presence of phenol red may interfere with results. In addition, the cultured cell line may not secrete sufficient ALT to be detected. It would be up to the end user to determine suitability.

      Helpful?

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