RAB0610

Human APOB / Apolipoprotein B-100 ELISA Kit

• Synonym(s): Apo B-100, Apolipoprotein B-100
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... APOB(338)
• Input: sample type serum; sample type plasma; sample type cell culture supernatant(s)
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable
• input: sample type serum; sample type plasma; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 2.5 ng/mL; standard curve range: 2.458-600 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... APOB(338)

Description

General description

The ApoB ELISA kit provides for the Quantitative measurement of ApoB-100 in Cell Culture Supernatants, Plasma and Serum.
The APOB (apolipoprotein B) gene is mapped to human chromosome 2p24.1. Apob protein is an amphipathic protein that exists as α- helices and β-sheets. Apob is present in the major lipoproteins : chylomicrons, low density lipoproteins and very low density lipoprotein.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Apob (apolipoprotein B) functions in the low-density lipoprotein cholesterol segregation. Increased APOB gene expression is observed in non-alcoholic fatty liver disease and contributes to its pathogenesis. Reduced Apob protein is indicated in familial hypobetalipoproteinemia.

Kit Components Also Available Separately

• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 10 - Combustible liquids