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Human TIMP-2 ELISA Kit

Name: Human TIMP-2 ELISA Kit
Brand: SIGMA

for serum, plasma, cell culture supernatant and urine

Synonym(s):

Metalloproteinase inhibitor 2 , TIMP-2, Tissue inhibitor of metalloproteinases 2

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About This Item

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... TIMP2(7077)

Input:

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

Pricing and availability is not currently available.

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Properties

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.6-1200 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TIMP2(7077)

Description

General description

The Human TIMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIMP-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human TIMP2

Application

For research use only. Not for use in diagnostic procedures.
Human TIMP-2 ELISA Kit has been used to measure tissue inhibitor of metalloproteinase-2 (TIMP-2) level in blood samples from Atherosclerotic renal artery stenosis (ARAS) patients.

Biochem/physiol Actions

Tissue inhibitor of metalloproteinase-2 (TIMP-2) plays a vital role in inhibition of matrix metalloproteinases (MMPs) and functions as a key modulator of extracellular matrix deposition and degradation in various physiological processes. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. TIMP-2 mediates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22. TIMP-2 functions as a biomarker for cell cycle arrest.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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This Item	RAB0467	RAB0466	RAB0473
Sigma-Aldrich RAB0472 Human TIMP-2 ELISA Kit	Sigma-Aldrich RAB0467 Human TIMP-1 ELISA Kit	Sigma-Aldrich RAB0466 Human TIMP-1 ELISA Kit	Sigma-Aldrich RAB0473 Mouse TIMP-2 ELISA Kit
Gene Information human ... TIMP2(7077)	Gene Information human ... TIMP1(7076)	Gene Information human ... TIMP1(7076)	Gene Information mouse ... Timp2(21858)
species reactivity human	species reactivity human	species reactivity human	species reactivity mouse
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 1.6-1200 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 30 pg/mL standard curve range: 24.69-18000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 40 pg/mL standard curve range: 24.69-18000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 80 pg/mL standard curve range: 0.0823-60 ng/mL
input sample type urine sample type cell culture supernatant(s) sample type serum sample type plasma	input sample type tissue lysate sample type cell lysate	input sample type cell culture supernatant(s) sample type urine sample type serum sample type plasma	input sample type cell culture supernatant(s) sample type plasma sample type serum
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable
packaging kit of 96 wells (12 strips x 8 wells)	packaging kit of 96 wells (12 strips x 8 wells)	packaging kit of 96 wells (12 strips x 8 wells)	packaging kit of 96 wells (12 strips x 8 wells)