P7002

Phospho(enol)pyruvic acid trisodium salt hydrate

≥97% (enzymatic), powder

• Synonym(s): 2-(Phosphonooxy)-2-propenoic acid trisodium salt, PEP
• Linear Formula: C₃H₂O₆PNa₃ · x H₂O
• CAS Number: 5541-93-5
• Molecular Weight: 233.99 (anhydrous basis)
• UNSPSC Code: 12352204
• PubChem Substance ID: 24898810
• NACRES: NA.32
• MDL number: MFCD00150734

Properties

• Product Name: Phospho(enol)pyruvic acid trisodium salt hydrate, ≥97% (enzymatic)
• Quality Segment: 200
• assay: ≥97% (enzymatic)
• form: powder
• solubility: water: 100 mg/mL, clear to slightly hazy, colorless to very faintly yellow
• storage temp.: −20°C
• SMILES string: [Na].OC(=O)C(=C)OP(O)(O)=O
• InChI: 1S/C3H5O6P.Na.H/c1-2(3(4)5)9-10(6,7)8;;/h1H2,(H,4,5)(H2,6,7,8);;
• InChI key: KZPUEZVJSLLABH-UHFFFAOYSA-N

Description

Application

Phospho(enol)pyruvic acid trisodium salt hydrate has been used in:

• several enzyme activity assays, such as PRPP synthetase (PRPPS) assay, ribokinase (RK) assay, adenine phosphoribosyltransferase (APRT) assay
• in the reaction mixture for enzyme kinetic assay to determine platelet-derived growth factor receptor α (PDGFRA) kinetic parameters
• as a component in buffer B to react with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP) for energy charge measurement

Biochem/physiol Actions

Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogenesis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

Phosphoenolpyruvic acid plays a major role in the initial step of glycolysis by acting as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)