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Medium 199

Name: Medium 199
Brand: SIGMA

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Synonym(s):

Cell growth medium, M199 Medium, TCM 199

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO₂): yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

to culture human mesothelial cells[1]
as a component of the holding medium for the collection of embryos from pluriparous Boer goat
in the preparation of spheroid-embedded collagen matrices[2]

Preparation Note

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

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Show Differences

1 of 1

This Item	M9163	M7528	M5017
Sigma-Aldrich M0650 Medium 199	Sigma-Aldrich M9163 Medium 199	Sigma-Aldrich M7528 Medium 199	Sigma-Aldrich M5017 Medium 199
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form powder
components NaHCO₃: no phenol red: yes sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes L-glutamine: no	components phenol red: yes HEPES: no sodium pyruvate: no Hanks’ salts (2% CO₂): yes L-glutamine: no NaHCO₃: no	components L-glutamine: no sodium pyruvate: no HEPES: 25 mM phenol red: yes NaHCO₃: yes Earle’s salts (5% CO₂): yes	components L-glutamine: yes phenol red: yes NaHCO₃: no sodium pyruvate: no Earle’s salts (5% CO₂): yes HEPES: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
M0650-100ML	04061833993811

Questions

Anonymous

2 years ago

If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?

1 answer
1. Technical Support
  
  2 years ago
  
  Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
  Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
  (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
  • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
  • While gently stirring the water, add 100 ml of 10X medium.
  • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
  • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
  • Bring medium to final volume with additional tissue culture grade water.
  • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
  • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
  
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