GL0010
Golgi Isolation Kit
sufficient for 50 g (tissue)
Synonym(s):
Golgi Kit, Isolation Kit for Golgi
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Pricing and availability is not currently available.
Quality Level
usage
sufficient for 50 g (tissue)
technique(s)
fractionation: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).
Application
Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.
Analysis Note
The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.
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This Item | |||
|---|---|---|---|
| technique(s) fractionation: suitable | technique(s) centrifugation: suitable | technique(s) - | technique(s) - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| usage sufficient for 50 g (tissue) | usage - | usage sufficient for 25 nuclei preparations (~1-10×107 cells/preparation) | usage - |
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL
Storage Class
10 - Combustible liquids
wgk
WGK 3
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| GL0010-1KT | 04061833654798 |