If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
38733
Laemmli Lysis-buffer
non smelling
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| Pack Size | SKU | Availability | Price |
|---|
About This Item
Quality Segment
form
liquid
composition
bromophenol blue, 0.004% , DTT, 400 mM , glycerol, 20% , SDS, 4% , TRIS, 0.125 M
pH
~6.8
suitability
in accordance for electrophoresis test
storage temp.
−20°C
Application
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 2-8°C |
| pH ~6.8 | pH 4.00±0.01 (25 °C) | pH 10.00±0.01 (25 °C) | pH 6.0 |
| suitability in accordance for electrophoresis test | suitability - | suitability - | suitability - |
| composition bromophenol blue, 0.004% , glycerol, 20% , TRIS, 0.125 M , DTT, 400 mM | composition - | composition - | composition - |
signalword
Danger
hcodes
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class
8B - Non-combustible corrosive hazardous materials
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 38733-5ML | 04061838047588 |
| 38733-5X2ML | 04061838084361 |
| 38733-2ML | 04061838236128 |
| 38733-1ML | 04061838253583 |
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?
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An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.
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What is the difference between the two Laemmli buffers S3401 and 38733?
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Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue. So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).
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Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?
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For most applications, it is considered to be a 2X solution.
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What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?
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The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol). Some people are very sensitive to the odor of 2-mercaptoethanol.
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