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3HBDB-RO

Roche

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II

Synonym(s):

3-Hydroxybutyrate Dehydrogenase (3-HBDH), 3-HBDH

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About This Item

UNSPSC Code:
12352204
EC Number:
1.1.1.30 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
Concentration:
5 mg/mL
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form

suspension

specific activity

~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)

packaging

vial of 2 mL (10127833001), vial of 5 mL (10127841001)

manufacturer/tradename

Roche

concentration

5 mg/mL

impurities

<0.1% LDH, <5% MDH

optimum pH

6.2-6.9(for reduction), 7-9(for oxidation)

shipped in

wet ice

storage temp.

2-8°C

General description

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.[1]

Application

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.

Biochem/physiol Actions

3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-­hydroxybutyrate.[2] In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.[3]

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Stabilizers: NADH, Ca2+

Analysis Note

Contaminants: <0.1% LDH, <5% MDH

Other Notes

For life science research only. Not for use in diagnostic procedures.

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This Item
H8509H9408GDH-RO
3-Hydroxybutyrate Dehydrogenase (3-HBDH) suspension, ~3&#160;units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II

Roche

3HBDB-RO

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

&#946;-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides Type V, lyophilized powder, 250-750&#160;units/mg protein

Sigma-Aldrich

H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

&#946;-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei lyophilized powder, &#8805;200&#160;units/mg protein

Sigma-Aldrich

H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

Glycerol-3-Phosphate Dehydrogenase (GDH) from rabbit muscle

Roche

GDH-RO

Glycerol-3-Phosphate Dehydrogenase (GDH)

specific activity

~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)

specific activity

250-750 units/mg protein

specific activity

≥200 units/mg protein

specific activity

170 U/mg (Approximately 170 U/mg at +25°C with DAP as the substrate (optimized conditions).), ~170 units/mg protein (At 25 °C with DAP as the substrate (optimized conditions).)

concentration

5 mg/mL

concentration

-

concentration

-

concentration

-

form

suspension

form

lyophilized powder

form

lyophilized powder

form

suspension

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

optimum pH

6.2-6.9(for reduction), 7-9(for oxidation)

optimum pH

-

optimum pH

-

optimum pH

8.0-8.5

impurities

<0.1% LDH, <5% MDH

impurities

-

impurities

-

impurities

-

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

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Certificates of Analysis (COA)

Lot/Batch Number
64483534
64483528
64483527
64483524
64483523

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Yoshitaka Nakajima et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 61(Pt 1), 36-38 (2006-03-02)
A recombinant form of D-3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) from Pseudomonas fragi has been crystallized by the hanging-drop method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P2(1)2(1)2, with unit-cell parameters a = 64.3, b
E B Worrall et al.
The Biochemical journal, 241(1), 297-300 (1987-01-01)
The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3-hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as
Fahimeh Khorsand et al.
IET nanobiotechnology, 7(1), 1-6 (2013-05-28)
Precise detection of 3-hydroxybutyrate (HB) in biological samples is of great importance for management of diabetic patients. In this study, an HB biosensor based on single-walled carbon nanotubes (SWCNTs)-modified screen-printed electrode (SPE) was developed to determine the concentration of HB

Global Trade Item Number

SKUGTIN
1012784100104061838667533
1012783300104061838667526