3HBDB-RO

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II

• Synonym(s): 3-Hydroxybutyrate Dehydrogenase (3-HBDH), 3-HBDH
• Enzyme Commission number: 1.1.1.30 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• Quality Level: 100
• form: suspension
• specific activity: ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
• packaging: vial of 2 mL (10127833001); vial of 5 mL (10127841001)
• manufacturer/tradename: Roche
• concentration: 5 mg/mL
• impurities: <0.1% LDH; <5% MDH
• optimum pH: 6.2-6.9(for reduction); 7-9(for oxidation)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.

Application

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.

Biochem/physiol Actions

3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-­hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.

Quality

Contaminants: <0.1% LDH, <5% MDH

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Stabilizers: NADH, Ca²⁺

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available