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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1F11, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 132.47 kDa
observed mol wt ~155 kDa
物種活性
rat
物種活性（以同源性預測）
mouse, bovine, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
C-terminus
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1F11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Metabotropic glutamate receptor 5 (mGluR5). It targets an epitope within 23 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 23 amino acids the C-terminal region of human Metabotropic glutamate receptor 5 (mGluR5).
應用
Quality Control Testing
Evaluated by Western Blotting in Rat brain microsome.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mGluR5 in Rat brain microsome.
Tested applications
Affinity Binding Assay: A representative lot of this antibody bound mGluR5 peptide with a KD of 2.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in E18 rat cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected mGluR5 in rat brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Rat brain microsome.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mGluR5 in Rat brain microsome.
Tested applications
Affinity Binding Assay: A representative lot of this antibody bound mGluR5 peptide with a KD of 2.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in E18 rat cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected mGluR5 in rat brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This rabbit recombinant monoclonal Anti-mGluR5, clone 1F11 ZooMAb® Rabbit Monoclonal, Cat. No. ZRB1270 is used in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry and Affinity Binding Assay, for the detection of mGluR5.
標靶描述
Metabotropic glutamate receptor 5 (UniProt: P41594; also known as mGluR5) is encoded by the GRM5 (also known as GPRC1E, MGLUR5) gene (Gene ID: 2915) in human. Glutamate receptors can be grouped into ionotropic receptors and metabotropic receptors. Excitotoxic effects of glutamate analogs are primarily achieved through the activation of the ionotropic receptors. Glutamate can also activate metabotropic receptors that couple GTP-binding proteins to intracellular second messengers. These receptors are distributed throughout the central nervous system and are located at synaptic as well as extra-synaptic sites in neurons and glia. Metabotropic receptors contain a large extracellular N-terminal domain known as the Venus flytrap domain (VFD) that contains the glutamate-binding site and has two lobes that sit one atop the other and bind glutamate in a cleft between the two lobes. The VFD dimer can exist in open-open, open-closed, or in closed-closed state. The open-open state is an inactive conformation and is stabilized by antagonists. At least eight metabotropic glutamate receptors (mGluR1-8) have been reported. They are classified into three groups based on their sequence homology, agonist pharmacology, and coupling to intracellular transduction mechanisms. The mGluR1 and mGluR5 belong to group I and these receptors are coupled to post-synaptic inositol phosphate metabolism. They couple to Gq/G11 and activate phospholipase Cβ that hydrolyzes phosphoinositides to generate inositol 1, 4, 5-trisphosphate (IP3) and diacyl- glycerol (DAG), both of which participate in the activation of protein kinase C. Activation of Group I receptors leads to depolarization and increases neuronal excitability. mGluR1 is present at high levels in the cerebellum and thalamus and mGluR5 is found in superficial cortical layers and the olfactory bulb. The activation of mGluR1 generates a single spike in Ca2+ concentrations, whereas mGluR5 activation leads to Ca2+ oscillations via phosphorylation/dephosphorylation cycles. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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