推荐产品
生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
5G18, recombinant monoclonal
产品线
ZooMAb® learn more
表单
lyophilized
分子量
calculated mol wt 26.42 kDa
observed mol wt ~35 kDa
种属反应性
human, mouse
种属反应性（根据同源性预测）
monkey
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技术
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG
表位序列
C-terminus
Protein ID登记号
UniProt登记号
环保替代产品分类
, Aligned
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 5G18 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Flt3 ligand. It targets an epitope within 25 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal, cytoplasmic domain of human Flt3 ligand.
应用
Quality Control Testing
Evaluated by Western Blotting in Raw264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flt3 ligand in Raw264.7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Flt3 ligand in HeLa and MCF-7 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Flt3 ligand peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Flt3 ligand in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Raw264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flt3 ligand in Raw264.7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Flt3 ligand in HeLa and MCF-7 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Flt3 ligand peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Flt3 ligand in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Flt3 ligand, clone 5G18 ZooMAb®, Cat. No. ZRB1096, is a recombinant Rabbit monoclonal antibody that specifically targets Flt3 ligand and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
目标描述
Fms-related tyrosine kinase 3 ligand (UniProt: P49771; also known as Flt3 ligand, Flt3L, FL, SL cytokine) is encoded by the FLT3LG gene (Gene ID: 2323) in human. Flt3-ligand is an important regulator of hematopoiesis that synergizes with a number of colony-stimulating factors and interleukins. It is expressed on myeloid, lymphoid, and dendritic cell progenitors. Expression of its receptor is restricted only to hematopoietic progenitors, early B cells and myeloid cells. Binding of Flt3-ligand to its receptor (Flt3) leads to receptor homodimerization and subsequent conformational changes that result in phosphorylation of its tyrosine kinase domains. This leads to rapid activation of receptor, its internalization, downstream actions, and degradation. Administration of Flt-3 ligand to mice is shown to cause a significant expansion of both CD8 + and CD8 dendritic cells at multiple sites, including spleen, lymph nodes, thymus, liver, lung, and bone marrow. Three isoforms of Flt3 have been described that are produced by alternative splicing. Isoform one is a single-pass type I membrane protein, isoform 2 is a homodimeric secreted or extracellular protein, and isoform 3 lacks the first 82 amino acids. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tsapogas, P., et al. (2017). Int. J. Mol. Sci. 18(6): 1115).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
商品
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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