跳转至内容
Merck

T3413

Sigma-Aldrich

Monoclonal Anti-Tenascin antibody produced in rat

clone MTn-12, ascites fluid

别名:

Anti-Tenascin-N

登录查看公司和协议定价


About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rat

品質等級

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

MTn-12, monoclonal

包含

15 mM sodium azide

物種活性

mouse

技術

immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:200 using unfixed, frozen tissue sections of mouse intestine
western blot: suitable

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

mouse ... Tnn(329278)

一般說明

Monoclonal Anti-Mouse Tenascin (rat IgG1 isotype) is derived from the MTn-12 hybridoma1 produced by the fusion of rat myeloma cells and splenocytes from a Lou rat immunized with partially purified mouse tenascin. Human tenascin has three subunits of 190, 200 and 220 kDa. Tenascin has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer, depending on species, the molecular weights of the subunits range from 190 to 320 kDa.
Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).
The tenascin molecule is a disulfide-linked hexamer; depending on species, the molecular weights of the subunits range from 190 to 320 kDa. In the mouse, two major subunits of tenascin with an apparent molecular weight of 210 and 260 kDa have been described. The shorter polypeptide predominates during earlier developmental stages and the larger polypeptide appears later in the embryonic gut and especially in the adult intestine. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence which may function in cell adhesion and it seems likely that tenascin mediates cell attachment through an RGD dependent integrin receptor.

特異性

The antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells.

免疫原

partially purified mouse tenascin.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Monoclonal Anti-Mouse Tenascin antibody may be used for the localization of tenascin and to study of the role of tenascin in epithelial-mesenchymal interactions using various immunochemical assays including ELISA, immunoblot, dot blot and immunohistology.
Monoclonal Anti-Tenascin antibody produced in rat has been used in:
  • Enzyme linked immunosorbent assay (ELISA)
  • Dot blot.
  • Immunoblotting
  • Fluorescence microscopy and immunostaining
  • Immunofluorescence
  • Immunohistochemistry

生化/生理作用

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence motif which may function in cell adhesion and may be recognized by integrin receptor.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Clinical impact and functional aspects of tenascin-C expression during glioma progression
Herold-Mende C, et al.
International Journal of Cancer. Journal International Du Cancer, 98(3), 362-369 (2002)
Transgenic overexpression of the alpha7 integrin reduces muscle pathology and improves viability in the dyW mouse model of merosin-deficient congenital muscular dystrophy type 1A
Doe JA, et al.
Journal of Cell Science, 124(13), 2287-2297 (2011)
Developmental changes in patterns of expression of tenascin-C variants in the human cornea
Maseruka H, et al.
Investigative Ophthalmology & Visual Science, 41(13), 4101-4107 (2000)
Anna Meuronen et al.
Respiratory research, 12, 2-2 (2011-01-06)
Asthma leads to structural changes in the airways, including the modification of extracellular matrix proteins such as tenascin-C. The role of tenascin-C is unclear, but it might act as an early initiator of airway wall remodelling, as its expression is
The Expression and Possible Functions of Tenascin-W During Development and Disease
Tucker RP and Degen M
Frontiers in Cell and Developmental Biology, 7, 53-53 (2019)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门