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Merck

T2551

Sigma-Aldrich

抗肌腱蛋白单克隆抗体 小鼠抗

clone BC-24, ascites fluid

别名:

Anti-Tenascin-N

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

BC-24, monoclonal

包含

15 mM sodium azide

物種活性

human

技術

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using protease-digested sections of adult human tissue.
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
radioimmunoassay: suitable
western blot: suitable

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... TNN(63923)

一般說明

Monoclonal Anti-Human Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Tenascin (TN) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. Human TN has 3 subunits of 190, 200 and 220 kD. TN has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. The expression of TN is associated with development and growth, both normal and pathological, with restricted distribution in normal adult tissue. It is synthesized by fibroblasts, chondroblasts, osteocytes, smooth muscle cells and glial cells. Human and chicken TN contain an RGD sequence which may function in cell adhesion and it seems likely that TN mediates cell attachment through an RGD-dependent integrin receptor. Mouse TN does not contain an RGD sequence in the third type III repeat implicated in cell attachment, or in any other positions.

特異性

The antibody recognizes an epitope within the N-terminal EGF-like (EGF-L) sequence of the molecule. It recognizes all human tenascin isoforms.

免疫原

human tenascin.

應用

Monoclonal Anti-Tenascin antibody produced in mouse has been used for immunohistochemistry.
Mouse monoclonal clone BC-24 anti-Tenascin antibody is an essential tool for the localization, identification and studies on the role of tenascin in epithelial-mesenchymal and neuronal-glial interactions.

生化/生理作用

Tenascin (TN) is up-regulated by epithelial transforming growth factor β (TGF-β) in mesenchyme. TN in the stroma of cancerous tissue contributes to tumorigenesis and progression of metastasis. High molecular weight TN isoform, a stromal marker of breast cancer, over-expresses implicating cell proliferation and invasion. TN has a potential role in tissue restoration. Intracytoplasmic TN immunoreactivity has been detected in malignant melanomas and in lung carcinomas. TN expression, when reacted with monoclonal antibody, determines its localization in tumor cells and its association with epithelial-mesenchymal and neuronal-glial cell interactions.

外觀

This product is supplied as ascites fluid containing 15 mM sodium azide

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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S F Masre et al.
Oncogene, 36(18), 2529-2542 (2016-12-20)
To study ROCK2 activation in carcinogenesis, mice expressing 4-hydroxytamoxifen (4HT)-activated ROCK2 (K14.ROCKer) were crossed with mice expressing epidermal-activated rasHa (HK1.ras1205). At 8 weeks, 4HT-treated K14.ROCKer/HK1.ras1205 cohorts exhibited papillomas similar to HK1.ras1205 controls; however, K14.ROCKer/HK1.ras1205 histotypes comprised a mixed papilloma/well-differentiated squamous
Allegra G Hawkins et al.
Molecular & cellular proteomics : MCP, 17(5), 901-912 (2018-02-02)
Tumor: tumor microenvironment (TME) interactions are critical for tumor progression and the composition and structure of the local extracellular matrix (ECM) are key determinants of tumor metastasis. We recently reported that activation of Wnt/beta-catenin signaling in Ewing sarcoma cells induces
Tenascin-C in primary malignant melanoma of the skin
Ilmonen S, et al.
Histopathology, 45(4), 405-411 (2004)
Tenascin-C induced signaling in cancer
Orend G and Ruth CE
Cancer Letters, 244(2), 143-163 (2006)
Expression of tenascin-C in various human brain tumors and its relevance for survival in patients with astrocytoma
Leins A, et al.
Cancer, 98(11), 2430-2439 (2003)

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