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等級
BioPerformance Certified
for molecular biology
品質等級
形狀
solution
濃度
1 M
技術
cell culture | mammalian: suitable
雜質
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
pH值
7.8
有用的pH值範圍
7.0-9.0
吸收
≤0.05 at 290 at 40%
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相关类别
一般說明
三羟甲基氨基甲烷(Tris)是生物科学中常用的缓冲剂,其pH缓冲范围介于7.0 - 9.0之间。在溶液中,它对金属的结合作用微不足道。Tris主要与乙二胺四乙酸(EDTA)结合使用,尤其适用于电泳研究。它的低离子迁移率在毛细管电色谱中也有应用。
一系列 TRIZMA 碱和 TRIZMA HCl 的预混合溶液,为 Tris 缓冲液提供常用的 pH 值。不必混合或调节 pH 值。保证精确度 ± 0.1 个 pH 单位。
應用
Trizma®盐酸溶液用于:
- 作为裂解缓冲液的组分,分解人胚肾(HEK) 293细胞
- 、10X地辛高(DIG)脱氧核苷三磷酸(dNTP)切口平移标记缓冲液及tris EDTA和氯化钠(TEN)缓冲液中
- 制备Tris、醋酸和乙二胺四乙酸(EDTA) (1x TAE)缓冲液并用作10X凝胶上样染料的组分
法律資訊
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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