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Merck
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T2194

Sigma-Aldrich

Trizma® 盐酸溶液

pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

别名:

Tris 盐酸盐溶液

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About This Item

UNSPSC代码:
12161700
NACRES:
NA.25
价格与库存信息目前不能提供

等级

BioPerformance Certified
for molecular biology

质量水平

200

表单

solution

浓度

1 M

技术

cell culture | mammalian: suitable

杂质

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH值（酸碱度）

7.4

有效pH范围

7.0-9.0

吸附

≤0.05 at 290 at 40%

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相关类别

一般描述

一系列 TRIZMA 碱和 TRIZMA HCl 的预混合溶液，为 Tris 缓冲液提供常用的 pH 值。不必混合或调节 pH 值。保证精确度 ± 0.1 个 pH 单位。

应用

Trizma ® 盐酸盐溶液曾用于：
  • 制备细胞裂解缓冲液 [1] [2]
  • 作为洗涤缓冲液和 RNase mix 1 的组分，用于制备 5′-用于 CdS 合成的溶解镉或 S 2- 载微反应器的双末端测序 [3]
  • 的完整 cDNA[4]

法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000398891
0000398891
0000398892
0000398892
SLCR1844

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RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5?-Complete cDNAs
Batut P and Gingeras TR
Current Protocols in Molecular Biology, 104(1), 25B-211 (2013)
Lab-on-a-micromotor: catalytic Janus particles as mobile microreactors for tailored synthesis of nanoparticles
Pacheco M, et al.
Chemical Science, 9(42), 8056-8064 (2018)
Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
Smith T and Phyu S
Bio-protocol, 6(22) (2016)
Matthieu Dos Santos et al.
STAR protocols, 2(3), 100694-100694 (2021-08-13)
Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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