SRE0006

胸苷磷酸化酶，重组 来源于大肠杆菌

recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL

• 别名: 胸苷：正磷酸脱氧-D-核糖基转移酶
• CAS号: 9030-23-3
• EC 号: 2.4.2.4 (BRENDA, IUBMB)
• MDL编号: MFCD00132426
• UNSPSC代码: 12352204
• NACRES: NA.54

属性

• 重组: expressed in E. coli
• 质量水平: 400
• 表单: buffered aqueous solution
• 浓度: ≥500 units/mL
• 技术: inhibition assay: suitable
• 颜色: colorless to yellow
• 溶解性: soluble; water: soluble
• NCBI登记号: NP_418799.1
• UniProt登记号: P07650
• 应用: diagnostic assay manufacturing
• 运输: wet ice
• 储存温度: 2-8°C
• 基因信息: Escherichia coli ... deoA(948901)

说明

一般描述

Research area: CELL SIGNALING

The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

应用

Thymidine phosphorylase has been used:

• in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy.[1]
• in a study to investigate implications for the clinical efficacy of nucleoside analogues.[2]

生化/生理作用

一种对胸腺嘧啶可逆性转化为胸腺嘧啶进行催化的酶。 胸苷磷酸化酶是嘧啶核苷挽救途径的一部分。

一种对胸腺嘧啶可逆性转化为胸腺嘧啶进行催化的酶。胸苷磷酸化酶是嘧啶核苷挽救途径的一部分。该途径允许嘧啶碱基被回收用于核苷酸生物合成，而1-磷酸戊糖被转化为磷酸戊糖分流和糖酵解的中间体。 大肠埃希菌胸苷磷酸化酶与人序列具有40%的序列同源性，已发现该序列与血管生成剂血小板衍生的内皮生长因子相同。 在鸡绒膜尿囊膜测定中，纯化的大肠埃希菌酶已显示出刺激血管生长的作用。

Thymidine phosphorylase catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the thymidine salvage pathway [3] and pyrimidine nucleoside salvage pathway.[4] This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis.[5] The enzyme inhibits apoptosis and induces angiogenesis thereby promoting tumor growth and metastatic process. [4] Moreover, thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

单位定义

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

制备说明

Cloned from E. coli and produced in overexpressing E. coli

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable