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Merck

S0937

Sigma-Aldrich

Sucrose Phosphorylase

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

别名:

SPase, 二糖葡糖基转移酶, 蔗糖转葡糖基酶,

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

重组

expressed in E. coli

表单

lyophilized powder

比活

≥45 units/mg solid

分子量

56 kDa by SDS-PAGE

运输

wet ice

储存温度

−20°C

一般描述

研究领域:细胞信号传导
蔗糖磷酸化酶属于糖苷水解酶GH13家族。它由四个结构域组成,包括葡萄糖异头碳结合位点和糖苷结合位点。活性位点残基包括Asp192和Glu232。它主要由双歧杆菌和乳酸杆菌产生。交联的蔗糖磷酸化酶聚集体具有热稳定性,可用于工业糖基化催化。

应用

蔗糖磷酸化酶已用于测定小麦植株中的蔗糖 和蔗糖氢的生产。
蔗糖磷酸化酶已用于:

  • 评估稳定、无味且呈粉末状的呋喃酮糖苷的酶促合成。
  • 研究与羧酸化合物的新型转糖基化反应。
  • 用于小麦植株中的蔗糖测定和蔗糖制氢。

生化/生理作用

蔗糖磷酸化酶催化蔗糖(α-D-吡喃葡萄糖基-1,2-β-D-呋喃果糖苷)的可逆转化和将磷酸盐催化成D-果糖和α-D-葡萄糖1-磷酸。此反应在通过蔗糖代谢产生至关重要的葡萄糖组分方面起着关键作用。(1)

单位定义

在 25°C和pH 7.6下,一个单位每分钟将从蔗糖产生 1.0μ摩尔 D 果糖,同时相应地将NADP还原为NADPH。

外形

含有作为稳定剂的蔗糖。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme
WangM, et al
Microbial cell factories, 17(1), 23-23 (2018)
Mario Mueller et al.
FEBS letters, 581(20), 3814-3818 (2007-07-31)
Site-directed mutagenesis was used to examine the specificity of Leuconostoc mesenteroides sucrose phosphorylase for utilization of fructose and phosphate as leaving group/nucleophile of the reaction. The largest catalytic defect in Arg(137)-->Ala (approximately 60-fold) and Tyr(340)-->Ala (approximately 2500-fold) concerned phosphate dependent
Thornthan Sawangwan et al.
Organic & biomolecular chemistry, 7(20), 4267-4270 (2009-10-02)
Regioselective glucosylation of R-glycerate catalysed by sucrose phosphorylase in the presence of sucrose as the donor substrate provided the natural compatible solute (R)-2-O-alpha-D-glucopyranosyl glycerate with complete regioselectivity in an optimised synthetic yield of 90% R-glycerate converted and a concentration of
A Kasperowicz et al.
Journal of applied microbiology, 107(3), 812-820 (2009-03-27)
To verify the taxonomic affiliation of bacterium Butyrivibrio fibrisolvens strain A from our collection and to characterize its enzyme(s) responsible for digestion of sucrose. Comparison of the 16S rRNA gene of the bacterium with GenBank showed over 99% sequence identity
Structural rearrangements of sucrose phosphorylase from Bifidobacterium adolescentis during sucrose conversion
Mirza O, et al.
The Journal of Biological Chemistry (2006)

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