推荐产品
一般描述
凝血酶 CleanCleave 试剂盒包含通过固定牛凝血酶产生的 50％ 凝血酶 - 琼脂糖悬浮液，用于切割重组融合蛋白
应用
用于从含有凝血酶识别序列的重组融合蛋白切割标签。凝血酶 - 琼脂糖与在各种类型的表达系统中表达的重组蛋白相容。
特点和优势
- 在短短 2 小时内快速、有效地裂解
- 凝血酶与琼脂糖共价结合，易于除去。
- 在 4°C 到 37°C 的温度范围内，以及在广泛的 pH 和离子强度范围内，强裂解反应是有效的。
- 即使在 0.1％Triton™，1M 尿素或 5mM EDTA 存在下，也能裂解标签
- 凝血酶 - 琼脂糖是可重复使用的
数量
200 μl 50％ 的凝血酶 - 琼脂糖浆液裂解 >85% 的 1mg 融合蛋白。
法律信息
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
仅试剂盒组分
产品编号
说明
- Thrombin Cleavage Buffer, 10× 10 mL
相关产品
产品编号
说明
价格
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Claudia H Huichalaf et al.
Human molecular genetics, 28(12), 2014-2029 (2019-02-13)
An early hallmark of Alzheimer's disease is the accumulation of amyloid-β (Aβ), inspiring numerous therapeutic strategies targeting this peptide. An alternative approach is to destabilize the amyloid beta precursor protein (APP) from which Aβ is derived. We interrogated innate pathways
Linda J Urbanski et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1834-1839 (2020-09-26)
This paper presents the production and kinetic and inhibitory characterisation of β-carbonic anhydrase from the opportunistic bacterium Staphylococcus aureus (SauBCA). From the eight different carbonic anhydrase (CA) families known to date, humans have only the α-form, whereas many clinically relevant
Robin Levy et al.
Biomolecules, 9(3) (2019-03-06)
The disordered p53 transactivation domain (p53TAD) contains specific levels of transient helical secondary structure that are necessary for its binding to the negative regulators, mouse double minute 2 (Mdm2) and MdmX. The interactions of p53 with Mdm2 and MdmX are
Bhavna Chawla et al.
PloS one, 7(3), e33138-e33138 (2012-03-23)
Deoxyhypusine hydroxylase (DOHH) catalyzes the final step in the post-translational synthesis of an unusual amino acid hypusine (N(€)-(4-amino-2-hydroxybutyl) lysine), which is present on only one cellular protein, eukaryotic initiation factor 5A (eIF5A). We present here the molecular and structural basis
Andrea Angeli et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 758-763 (2021-03-16)
We report the first activation study of the β-class carbonic anhydrase (CA, EC 4.2.1.1) encoded in the genome of the protozoan pathogen Trichomonas vaginalis, TvaCA1. Among 24 amino acid and amine activators investigated, derivatives incorporating a second carboxylic moiety, such
商品
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
实验方案
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门