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生物源
bovine plasma
形狀
lyophilized powder
比活性
40-300 NIH units/mg protein (biuret)
分子量
heavy chain ~33 kDa
light chain ~5 kDa
成份
Protein, 40-60%
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
cow ... F2(280685)
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一般說明
凝血酶是止血相关的最终凝血蛋白酶,对促凝血和抗凝血作用都有所促进。
應用
凝血酶可用于含有可接近的凝血酶识别位点以除去亲和标签的重组融合蛋白的位点特异性切割。凝血酶已用于一项对从脓毒性休克患者中持续性低凝状态进行评估的研究中。
生化/生理作用
丝氨酸蛋白酶,选择性断开纤维蛋白原中的精氨酸-甘氨酸键,形成纤维蛋白以及纤维蛋白肽 A 和 B。
單位定義
活性以NIH单位表示,通过与NIH凝血酶参考标准品直接对比确定。
外觀
含有氯化钠和Tris-HCl,pH 7.0的冻干粉
準備報告
用牛脑促凝血酶原激酶进行激活。
分析報告
基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆(由血浆与等量生理盐水混合制得)作为底物,以及 0.1mL 凝血酶样品(在含 1% 白蛋白缓冲液中保持稳定)。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。
其他說明
查看更多关于 凝血酶的信息, 请访问 www.sigma-aldrich.com/enzymeexplorer。
基底
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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