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Merck

P9577

Sigma-Aldrich

PCR 50 - 2,000 bp 标记物

for electrophoresis of PCR fragments

别名:

50-2000 bp ladder, PCR ladder for agarose gel electrophoresis, PCR marker for agarose gel electrophoresis, RTU PCR DNA ladder, RTU PCR DNA marker, ready to use DNA ladder, ready to use DNA marker

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About This Item

分類程式碼代碼:
41105337
NACRES:
NA.25

形狀

liquid

用途

sufficient for 50 uses

適合性

suitable for electrophoresis (DNA)

儲存溫度

−20°C

一般說明

Sigma′s PCR 50-2,000 bp标记包含8个dsDNA片段,间隔50-2,000个碱基,间隔均匀。 可以将5μl梯子直接装在琼脂糖或聚丙烯酰胺凝胶上的单个泳道中。

應用

PCR 50 - 2,000 bp标记物可用作DNA凝胶电泳的标记物。

成分

Sigma′s PCR 50 - 2,000 bp标记物为两个试管:
  • 1瓶(250 μl体积)PCR标记物
  • 1瓶6×上样缓冲液(250 μl体积),用于稀释样品

其他說明

在可预测的间隔(阶梯)双链DNA片段中包含8个条带,50-2,000 bp。
为获得最佳分离度,推荐琼脂糖凝胶浓度为 2.0%。

儲存類別代碼

10 - Combustible liquids

個人防護裝備

Eyeshields, Gloves


分析证书(COA)

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Undergraduate virology exercises demonstrate conventional and real-time PCR using commercially available HIV primers and noninfectious target.
Sulzinski MA, et al.
Biochemistry and Molecular Biology Education, 37, 232-235 (2009)

商品

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

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实验方案

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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