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生物源
bacterial (Pseudomonas spp.)
形狀
lyophilized powder
比活性
≥3 units/mg solid
分子量
~700 kDa
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
原儿茶酸3,4-双加氧酶属于非血红素铁家族酶,其活性位点含Fe3+。
應用
来自假单胞菌属的原儿茶酸3,4-双加氧酶(PCD)在与对羟基苯甲酸羟化酶偶联后可用于酶法测定胆碱酯酶。在单分子实验中可改善有机荧光团稳定性 ,也可用于研究根瘤菌中原儿茶酸的代谢。
该酶被用于与原儿茶酸(PCA)和Trolox一起产生氧清除系统。该酶的非血红素铁中心可催化PCA和分子氧转化为β-羧基-顺,顺-粘康酸,同时抗氧化剂Trolox抑制花青染料的缓慢闪烁和光漂白。在单分子运动试验中,该酶与DMB-BSA(动力蛋白运动缓冲液-BSA)、ATP和原儿茶酸一起被用于制备成像缓冲液。
生化/生理作用
原儿茶酸3,4-双加氧酶催化3,4-二羟基苯甲酸(原儿茶酸)降解为β-羧基-顺,顺-粘康酸。
物理性質
结构:非血红素铁蛋白
抑制剂:Ag+、Hg++、 PCMB
最佳pH9.0
最佳温度:60-65℃
pH稳定性:pH 7.0-9.0(25℃,72 h)
热稳定性:低于50℃ (pH 6.0,1 h)
抑制剂:Ag+、Hg++、 PCMB
最佳pH9.0
最佳温度:60-65℃
pH稳定性:pH 7.0-9.0(25℃,72 h)
热稳定性:低于50℃ (pH 6.0,1 h)
單位定義
在pH 7.5、37℃条件下,一单位酶每分钟可将1.0 μmM原儿茶酸氧化成3-羧基-顺,顺-粘康酸。
外觀
以冻干粉形式提供。
分析報告
缩二脲法测定蛋白
抑制劑
产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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