跳转至内容
Merck
所有图片(1)

主要文件

查看所有文档

H1009

Sigma-Aldrich

过氧化氢 溶液

Hydrogen peroxide solution

30 % (w/w) in H2O, contains stabilizer

登录查看公司和协议定价
所有图片(1)

About This Item

经验公式（希尔记法）:
H2O2
CAS号:
7722-84-1
分子量:
34.01
Beilstein:
3587191
MDL编号:
MFCD00011333
UNSPSC代码:
12352304
PubChem化学物质编号:
57654227
NACRES:
NA.77
价格与库存信息目前不能提供

蒸汽压

23.3 mmHg ( 30 °C)

方案

29.0-32.0% (titration)

表单

liquid

保质期

~2 yr

反应适用性

reagent type: oxidant

浓度

30 % (w/w) in H2O

技术

cell based assay: suitable
immunohistochemistry: suitable

密度

1.110 g/cm3

适用性

suitable for DNA precipitation

应用

general analytical
pharmaceutical

储存温度

2-8°C

SMILES字符串

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

过氧化氢(H2O2)是一种局部杀菌剂，在需氧细胞中作为有氧呼吸的副产物合成。它还可作为过氧化物酶体、线粒体或其他细胞区室中酶促反应的产物。

应用

过氧化氢溶液已用于：
  • 处理鼻上皮细胞，用于H2O2敏感性测定
  • 处理人永生化角质形成细胞系(HaCaT)和人黑色素瘤细胞系(GLL19)以诱导衰老
  • 孵育脑切片，进行溴脱氧尿苷 (BrdU)/NeuN染色
  • 细胞增殖测定，孵育体外实验所用的神经干细胞(NE-4C)

生化/生理作用

过氧化氢(H2O2)主要用于创口清洁。它可以通过氧化爆发和产生局部氧气来杀死病原体。H2O2可充当信号传导分子或第二信使。它以多种方式在基因表达中发挥关键作用。H2O2参与炎症调节。

特点和优势

《受体分类和信号转导》手册的 磷蛋白磷酸酶（酪氨酸） 页面有该化合物的介绍。想要浏览手册的其他页面， 请单击此处。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
如需了解生化试剂系列的更多信息，请填写此表。

可比产品

产品编号
说明
价格

相关产品

产品编号
说明
价格

289132

过氧化脲, 97%

通常也和此产品一起购买

产品编号
说明
价格

象形图

Corrosion
GHS05

警示用语：

Danger

危险声明

H318,H412

危险分类

Aquatic Chronic 3 - Eye Dam. 1

储存分类代码

5.1B - Oxidizing hazardous materials

WGK

WGK 1

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCX2387
STBL4103
MKCV5253
MKCV9876
SDBB1447

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

S V Lennon et al.
Cell proliferation, 24(2), 203-214 (1991-03-01)
Cell death may occur by either of two mechanisms: apoptosis or necrosis. Necrosis, the first type of cell death to be recognized, is an uncontrolled degenerative phenomenon invariably caused by noxious stimuli and is the result of irreversible failure of
Akitoshi Nakashima et al.
Scientific reports, 9(1), 11466-11466 (2019-08-09)
Pregnancy is a stress factor culminating into mild endoplasmic reticulum (ER) stress, which is necessary for placental development. However, excessive or chronic ER stress in pre-eclamptic placentas leads to placental dysfunction. The precise mechanisms through which excessive ER stress impacts
Shoichi Iriguchi et al.
Blood, 125(2), 370-382 (2014-10-29)
Although overexpression of T-bet, a master transcription factor in type-1 helper T lymphocytes, has been reported in several hematologic and immune diseases, its role in their pathogenesis is not fully understood. In the present study, we used transgenic model mice
Osamu Sakai et al.
Investigative ophthalmology & visual science, 56(1), 538-543 (2015-01-13)
The purpose of the present study was to investigate the role of glutathione peroxidase 4 (GPx4) in conjunctival epithelial cells. An immortalized human conjunctival epithelial cell line was used. Cells were transfected with catalase, GPx1, GPx4, SOD1, SOD2, or control
Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
查看所有相关文献

商品

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

实验方案

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

过氧化氢酶的酶学测定（EC 1.11.1.6）

过氧化氢酶催化过氧化氢生成水和氧气。该实验方案使用分光光度法测定过氧化氢酶活性。

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

过氧化物酶的酶学测定（EC 1.11.1.7）

该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。

查看所有结果
1 Review This action will navigate to reviews.

Questions

1–10 of 19 Questions  

  1. How much we need to add 30% H2O2 in 1mL of solution(water as solvent) to make100mM molarity?

    1 answer

    1. The molarity of this product is calculated to be 9.791 M based on a density of 1.11 g/mL, a formula weight of 34.01 g/mol, and a concentration of 30 w/w%. Here is the calculation:
      Molarity = Density x concentration x 1000 / formula weight = 1.11 x 0.3 x 1000 / 34.01 = 9.791 M.

      To make a 100 mM solution, use the Dilution Formula: C1V1 = C2V2 to find the volume V1 of the 30% H2O2 solution needed.
      C1 = 9.791 M (concentration of the stock solution)
      C2 = 0.1 M (desired concentration)
      V2 = 0.001 L + V1 (final volume, which is the sum of 1 mL of water and the volume of H2O2 added)

      Rearrange the formula to solve for V1:
      9.791 x V1 = 0.1 x (0.001 + V1)
      V1 = 0.1 x 0.001 / (9.791 - 0.1) = ~0.0000103 L = ~10.3 µL

      Therefore, approximately 10.3 µL of this product is required for a 100 mM H2O2 solution when mixed with 1 mL of water.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. What stabilizer was added? I can't find any information in the DSD or spec sheet.

    1 answer

    1. This product contains Dipicolinic acid at 5 – 10ppm as a stabilizer.

      Helpful?

  5. how can we determinate H2O2 concentration in case of 30% by titration , i found way to do this with sodium thiosulfate but they mentioned that is for 0.1% - 5 % con. - can i use it ?

    1 answer

    1. Using a procedure meant for lower concentrations of hydrogen peroxide may lead to less accurate results. Solvay chemicals has a method for determination of the concentration of hydrogen peroxide (20-70%) by titration with potassium permanganate solution: https://www.solvay.com/sites/g/files/srpend221/files/2019-10/H2O2%20Determination%20of%20Hydrogen%20Peroxide%20Concentration%20-20%20to%2070-.pdf

      Helpful?

  6. - CONTACT TIME 문의 해당 과산화수소 수용액 30% 이용하여 소독 진행 시 검증되어 있는 컨택타임이 있는지 문의 드립니다.

    1 answer

    1. Unfortunately, there is no verified contact time for the use of product H1009 in disinfecting.

      Helpful?

  7. Hi, I would like to get information on this product (H2O2 Cat # H1009-5ML) for Lot numbers MKCL6061 and MKCJ1034 to know their expiration dates. Please help me get this information. Thanks.

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately one year after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Please see the link below to access a specific lot or to review a sample Certificate:
      https://www.sigmaaldrich.com/product/sigma/H1009#product-documentation

      Helpful?

  8. how much we need to add 30% H2O2 in 7ml of solution(water as solvent) to make 5mM molarity....could you please tell with calculation?

    1 answer

    1. The molarity of this product will change slightly from lot to lot, based on the final concentration. The concentration range is 29 - 32 %. The molarity can be calculated using the basic equation (density in g/L x final percentage)/molecular weight. The molarity of a 30 % solution of this product can be calculated by the following:
      (1110 g/L X 0.30) / 34.01 = 9.79 M.

      The volume of product required to prepare 7 mL of 0.005 M H2O2 would be 3.6 ul. For ease of pipetting, it may be more practical to prepare a larger volume. For example:
      Use 3.6 uL to prepare 7 mL at 5 mM
      Use 7.7 uL to prepare 15 mL at 5 mM
      Use 10.3 uL to prepare 20 mL at 5 mM

      Alternatively, an intermediate working solution can be prepared that would require further dilution.
      To prepare a 1 M stock from the 9.79 M concentrate, a dilution may be prepared using the M1V1 =M2V2 formula.
      In this case, 1.02 mL of the 9.79 M stock is diluted to 10 mL to achieve a 1 M concentration.
      From the 1 M solution dilute 0.035 mL (35 ul) to a final volume of 7 mL to achieve a 5 mM concentration.

      Helpful?

  9. 30% is w/w ratio or its w/v ratio and can you give formula to calculate molarity of this H2O2 30% solution?

    1 answer

    1. As stated on the product detail page, this is hydrogen peroxide 30% w/w in H2O.

      The molarity of this product will change slightly from lot to lot, based on the final concentration. The concentration range is 29 - 32 %. The molarity can be calculated using the basic equation (density in g/L x final percentage)/molecular weight. The molarity of a 30% solution of this product can be calculated by the following:
      (1197 g/L X 0.30) / 34.01 = 9.79 M

      Helpful?

  10. How do I check the concentration of H2O2 for a solution that has been stored at 4 degrees Celsius for about a year? Do I check the absorbance at 240nm?

    1 answer

    1. The product concentration is 30 % (w/w) in H2O. This product has a shelf life of approximately 2 years. The concentration of this product is determined by iodometric titration. See the link below to review an application note provided by Mettler Toledo: https://www.mt.com/us/en/home/supportive_content/ana_chem_applications/titration/M860.html

      To further discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

1–10 of 19 Questions  

Reviews

Active Filters

  1. 1 Ratings-Only Review

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门