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P2922

Sigma-Aldrich

蛋白内切酶 Glu-C 来源于金黄色葡萄球菌  V8 来源于金黄色葡萄球菌 V8

Type XVII-B, lyophilized powder, 500-1,000 units/mg solid

别名:

V8 蛋白酶

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About This Item

CAS号:
66676-43-5
EC 号:
3.4.21.19 (BRENDA, IUBMB)
MDL编号:
MFCD01324998
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54
价格与库存信息目前不能提供

类型

Type XVII-B

质量水平

200

表单

lyophilized powder

比活

500-1,000 units/mg solid

分子量

29 kDa

纯化方式

chromatography

运输

wet ice

储存温度

−20°C

基因信息

Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044)

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应用

内切蛋白酶Glu-C（来自金黄色葡萄球菌 Staphylococcus aureus V8）已用于消化还原和烷基化环肽以生产线性片段。[1]
它可用于蛋白质选择性切割以便进行氨基酸序列测定[2]或肽谱分析。[3]
来自 Staphylococcus aureus菌株V8的内切蛋白酶Glu-C是一种丝氨酸蛋白酶，可用于蛋白质选择性切割，用来进行氨基酸序列确定或多肽图谱。 产品P2922用于线性化蝶豆叶子提取物[4]中的环状多肽。

生化/生理作用

在磷酸缓冲液中，Staphylococcus菌株V9蛋白酶特异性地切割多肽链天冬氨酸和谷氨酸残基的羧基侧。 当在碳酸氢铵缓冲液或醋酸铵中时，只切割谷氨酸残基的羧基侧。 该酶在pH4.0到7.8之间有最大活性。如果使用血红蛋白为底物，最大活性在pH4.0。 当酪蛋白为底物，最大活性在pH7.8。

单位定义

1单位酶可在pH7.8,37℃条件，每分钟水解1 μmole N-t-Boc--L-谷氨酸α-苯酯。[5]一单位相当于 ~0.004单位酪蛋白消化所需的酶量。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000408423
0000420156
SLCM2238
0000368425
0000368425

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Andrew Michael Frey et al.
Cell reports, 35(1), 108930-108930 (2021-04-08)
Staphylococcus aureus possesses ten extracellular proteases with mostly unknown targets in the human proteome. To assist with bacterial protease target discovery, we have applied and compared two N-terminomics methods to investigate cleavage of human serum proteins by S. aureus V8 protease
Shanshan Liu et al.
Amino acids, 48(4), 1059-1067 (2016-01-11)
Common yet often overlooked, deamidation of peptidyl asparagine (Asn or N) generates aspartic acid (Asp or D) or isoaspartic acid (isoAsp or isoD). Being a spontaneous, non-enzymatic protein post-translational modification, deamidation artifact can be easily introduced during sample preparation, especially
Jason M Gilmore et al.
Analytical and bioanalytical chemistry, 402(2), 711-720 (2011-10-18)
Protein phosphorylation is a reversible post-translational modification known to regulate protein function, subcellular localization, complex formation, and protein degradation. Detailed phosphoproteomic information is critical to kinomic studies of signal transduction and for elucidation of cancer biomarkers, such as in non-small-cell
Aaron G Poth et al.
The Journal of biological chemistry, 287(32), 27033-27046 (2012-06-16)
Cyclotides are a large family of plant peptides that are structurally defined by their cyclic backbone and a trifecta of disulfide bonds, collectively known as the cyclic cystine knot (CCK) motif. Structurally similar cyclotides have been isolated from plants within
Luca Fornelli et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 17(2), 486-497 (2011-01-06)
Tissue transglutaminase (tTGase) catalyzes both deamidation and transamidation of peptides and proteins by using a peptidyl glutamine as primary substrate. A precise consensus sequence for the enzyme is unknown and the ratio between deamidated and transamidated (or cross-linked) reaction products
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