跳转至内容
Merck
所有图片(2)

主要文件

查看所有文档

P2893

Sigma-Aldrich

JumpStart™ Taq ReadyMix™

Complete optimized reagent for hot-start PCR at 2X concentration

别名:

hot start PCR master mix, hot start master mix, hot start taq master mix, mutiplex PCR

登录查看公司和协议定价
所有图片(2)

About This Item

MDL编号:
MFCD00166026
UNSPSC代码:
41106300
NACRES:
NA.55

质量水平

200

表单

liquid

用途

sufficient for 100 reactions
 sufficient for 400 reactions

特点

Multiplex PCR
dNTPs included
hotstart

浓度

2.5 units/reaction (50 μL reaction volume)

技术

PCR: suitable

颜色

colorless

适用性

suitable for (quantitative PCR)

应用

agriculture

运输

wet ice

储存温度

−20°C

相关类别

一般描述

JumpStart™ Taq ReadyMix™是一种即用型2X预混液，其中含有JumpStart™ Taq DNA聚合酶、99％纯dNTP、反应缓冲液和JumpStart™抗体。Taq DNA聚合酶是一种抗体灭活的热启动酶。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。相对于手动热启动技术，这种抗体-酶复合物可轻松简便地准备，同时可降低污染风险。

应用

JumpStart™ Taq ReadyMix™ 已用于定量聚合酶链式反应(PCR)。此产品还可用于鉴定缺氧条件下的成纤维细胞表面腺苷受体(AR)类型。同时适用于：
  • 用于需要减少非特异性扩增的 PCR 扩增
  • 用于多重 PCR
  • 用于减少引物二聚体

特点和优势

  • 该预混液可确保反应间一致性
  • 缩短了准备时间，并减少了多个移液步骤造成的污染风险
  • 可最大限度减少非特异性扩增和污染
  • 提高了特异性和靶标产量
  • 减少了引物二聚体
  • 与手动或蜡质热启动方法相比，准备时间更短
  • 允许室温反应
  • 非常适合高通量定量PCR应用

包装

默认反应体积为50 μL

100个反应包装为1×2.5mL
400个反应包装为1×10mL

其他说明

JumpStart Taq ReadyMix是一种制备好的溶液，其结合了热启动PCR的性能优势和ReadyMix的便利性。该混合物包括JumpStart Taq DNA聚合酶、99％纯脱氧核苷酸、以及2x优化的反应浓缩液缓冲液。JumpStart Taq聚合酶是一种抗体灭活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量。由于JumpStart Taq聚合酶的聚合酶活性在PCR反应的第一个变性循环期间即可完全恢复，因而其与其他热启动方法（即化学灭活）不同，在循环前不需要预孵育步骤。热启动机制便于在室温进行设置，使得其成为高通量应用的理想选择。若需要制备50 μL反应，只需将25 μL ReadyMix添加至模板、引物和水中，最终反应体积为50 μL。
2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
准备50 μL反应物，只需将 25 μL ReadyMix™加入模板、引物和水，至终反应体积50 μL。
本品已通过定量PCR验证，但可能需要补充25 mM氯化镁溶液（货号M8787）、适宜的荧光探针以及（如果需要）内参染料（货号R4526）。请在www.sigma-aldrich.com/hotstart页面查看有关JumpStart Taq ReadyMix产品的更多详细信息。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：美国：US 8,404,464和US 7,972,828。 根据前述专利权利要求，购买本产品包含有限不可转让的免于诉讼豁免。
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

相关产品

产品编号
说明
价格

I9516

2-丙醇, for molecular biology, BioReagent, ≥99.5%

D8418

二甲基亚砜, for molecular biology

B7906

正丁醇, for molecular biology, ≥99%

M5904

矿物油, BioReagent, for molecular biology, light oil

C0549

氯仿:异戊醇 24:1, suitable for nucleic acid purification

B0300

甜菜碱 溶液, 5 M, PCR Reagent

W4502

水, Nuclease-Free Water, for Molecular Biology

D4184

JumpStart™ Taq DNA聚合酶, without MgCl2

T9424

TRI试剂®, For processing tissues, cells cultured in monolayer or cell pellets

D9307

JumpStart™ Taq DNA聚合酶, with MgCl2

D8187

JumpStart™ REDTaq® DNA Polymerase, Hot-start Taq enzyme with inert dye, 10X buffer included

P1107

JumpStart™ REDTaq® ReadyMix™ 反应混合物, for High-throughput PCR of complex templates

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000334667
0000334667
0000334665
0000334669
0000334665

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Koen Kole et al.
GigaScience, 6(10), 1-6 (2017-10-13)
Experience-dependent plasticity (EDP) is essential for anatomical and functional maturation of sensory circuits during development. Although the principal synaptic and circuit mechanisms of EDP are increasingly well studied experimentally and computationally, its molecular mechanisms remain largely elusive. EDP can be
Effects of A2BR on the biological behavior of mouse renal fibroblasts during hypoxia
Tang Jin, et al.
Molecular Medicine Reports, 11(6), 4397-4402 (2015)
Alex J McCarthy et al.
Infection and immunity, 87(5) (2019-03-06)
Gastrointestinal (GI) colonization of 2-day-old (P2) rat pups with Escherichia coli K1 results in translocation of the colonizing bacteria across the small intestine, bacteremia, and invasion of the meninges, with animals frequently succumbing to lethal infection. Infection, but not colonization
Diego Ottaviani et al.
Genome research, 18(11), 1778-1786 (2008-10-14)
The folding of chromatin into topologically constrained loop domains is essential for genomic function. We have identified genomic anchors that define the organization of chromatin loop domains across the human major histocompatibility complex (MHC). This locus contains critical genes for
Valerie F Boltz et al.
Retrovirology, 13(1), 87-87 (2016-12-22)
Although next generation sequencing (NGS) offers the potential for studying virus populations in unprecedented depth, PCR error, amplification bias and recombination during library construction have limited its use to population sequencing and measurements of unlinked allele frequencies. Here we report
查看所有相关文献

商品

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

查看所有结果

实验方案

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

相关内容

Modern Plant Breeding

Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.

Modern Plant Breeding

Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门