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B0300

甜菜碱溶液

Name: 甜菜碱 溶液
Brand: SIGMA

5 M, PCR Reagent

别名:

TMG, trimethylglycine

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About This Item

线性分子式:

(CH₃)₃N⁺CH₂COO⁻

CAS号:

107-43-7

分子量:

117.15

Beilstein:

3537113

MDL號碼:

MFCD00012123

分類程式碼代碼:

12352200

PubChem物質ID:

24891511

NACRES:

NA.52

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等級

PCR Reagent

品質等級

300

形狀

liquid

包裝

vial of 1.5 mL

濃度

5 M

技術

PCR: suitable

顏色

colorless

應用

agriculture

異物活動

DNase, RNase, none detected

儲存溫度

2-8°C

SMILES 字串

C[N+](C)(C)CC([O-])=O

InChI

1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3

InChI 密鑰

KWIUHFFTVRNATP-UHFFFAOYSA-N

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一般說明

甜菜碱也称为三甲基甘氨酸或N,N,N三乙基乙酸铵，是具有三个甲基的甘氨酸类似物。这种成分高度兼容聚合酶链式反应(PCR)缓冲液混合物。甜菜碱是一种PCR增强试剂，广泛用于提高PCR产物产量和特异性——尤其是在PCR扩增高GC含量的靶标，或PCR扩增形成二级结构、导致产量不佳的靶标过程中。甜菜碱可促进DNA链分离，并管理DNA中GC和AT对之间的DNA熔化温度(Tm)差异。它通过平衡GC-和AT-碱基对的贡献来稳定ds DNA。甜菜碱广泛用于优化多重和“长链且精确”的聚合酶链式反应（LA-PCR）。据报道，向PCR混合物中添加1.0-1.7M甜菜碱水溶液，可减少碱基对组成对DNA链解链的依赖性。

應用

甜菜碱溶液已被用作以下反应混合物的成分:

环介导等温扩增 (LAMP) 反应
用于基因组 DNA 扩增的聚合酶链反应
用于端粒长度分析的定量聚合酶链反应 (qPCR)
基因组 DNA 中 CGG 重复序列的 PCR 扩增
用于检测严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)的逆转录环介导的等温扩增(RT-LAMP)
DNA 定向 RNA 聚合酶 II 最大亚基(RPB2) 的 PCR 扩增
文库制备过程使用 Smartseq2 进行逆转录

包裝

每管1.5mL

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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甜菜碱溶液