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Merck
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NA0400

Sigma-Aldrich

GenElute™ HP 无内毒素质粒大提试剂盒

greener alternative

sufficient for 10 preparations

别名:

Gen Elute, GenElute 无内毒素质粒试剂盒

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 10 preparations

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

DNA extraction: suitable

環保替代類別

Aligned,

儲存溫度

15-25°C

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相关类别

一般說明

GenElute HP 无内毒素质粒大量制备试剂盒可以简单、快速地从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。该试剂盒采用了可快速澄清细菌裂解液的滤柱和可捕获质粒DNA的硅胶柱。配套的专利溶液可使质粒DNA结合到结合柱上，同时避免内毒素随之吸附。该技术使得用户可以维持一致的、低于 0.1 EU/μg 质粒DNA的内毒素水平。

制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。
我们致力于为您带来更加绿色的替代产品，这些产品遵守一项或多项绿色化学12项原则。相比标准DNA提取方法使用苯酚和氯仿，该产品是一种更安全的化学产品。

特點和優勢

  • 可纯化出多达1.2 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
  • 从沉淀细胞到获得纯化的质粒，仅需35分钟
  • 方便的真空形式

原則

通过离心分离法收集过夜的重组大肠杆菌培养液，然后通过改性SDS 碱裂解法进行处理。过滤澄清裂解液，然后加入针对无内毒素质粒制备而优化的结合溶液。随后，质粒捕获到硅胶膜上，同时避免内毒素随之吸附到膜上。通过两遍洗涤步骤，去除污染物。最后，在不含内毒素的水中洗脱结合所得的DNA。回收的质粒DNA主要以超螺旋形式存在。所含的基因组DNA和RNA均低于溴化丙啶染色琼脂糖凝胶电泳的检测水平。

其他說明

更多信息，请见www.sigma-aldrich.com/genelutehp。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Paul A Beare et al.
Journal of bacteriology, 191(5), 1369-1381 (2008-12-31)
Coxiella burnetii is a gram-negative obligate intracellular bacterium and the causative agent of human Q fever. The lack of methods to genetically manipulate C. burnetii significantly impedes the study of this organism. We describe here the cloning and characterization of
João M Furtado et al.
Investigative ophthalmology & visual science, 53(11), 6856-6862 (2012-09-07)
Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses the retina from the bloodstream. We investigated the dendritic cell as a potential taxi for T. gondii tachyzoites moving across the human retinal endothelium, and examined the participation of adhesion molecules
Keith Hansen et al.
Journal of visualized experiments : JoVE, (64)(64), e3304-e3304 (2012-06-27)
Genome editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Traditional gene editing methods such as chemical-based mutagenesis or random integration of DNA sequences confer indiscriminate genetic changes in an
Dale Cowley et al.
eLife, 4 (2015-09-04)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010
Ming-Wei Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13538-13543 (2008-09-04)
H5N1 influenza viruses have spread extensively among wild birds and domestic poultry. Cross-species transmission of these viruses to humans has been documented in over 380 cases, with a mortality rate of approximately 60%. There is great concern that a H5N1
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