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NA0310

Sigma-Aldrich

GenElute™ HP质粒大量制备试剂盒

greener alternative

sufficient for 25 purifications

别名:

GenElute™ HP质粒大量制备试剂盒

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.52
价格与库存信息目前不能提供

用途

sufficient for 25 purifications

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

DNA purification: suitable

环保替代产品分类

Aligned,

储存温度

15-25°C

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相关类别

一般描述

GenElute HP质粒大量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒采用了一种可快速清除裂解液的过滤针筒和一种适于真空或离心形式的硅胶结合柱。从150 mL Luria Broth (LB)培养基过夜培养物中，可分离出多达1.2 mg 的质粒DNA。请注意，实际得率取决于所用的菌株、质粒和培养基。

通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。通过两遍洗涤步骤，去除污染物。最后，在洗脱溶液(Tris-HCl)或水中洗脱结合所得的DNA。

回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接供限制性酶切、连接、测序、PCR、转化和转染等下游应用使用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。

应用

GenElute™ HP Plasmid Maxiprep试剂盒已用于分离质粒。[1]

特点和优势

  • 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
  • 可纯化出多达1.2 mg的高拷贝数质粒DNA
  • 真空或离心形式可灵活选择
  • 相对于其他快速试剂盒，所含的塑料成分更少，减少了废物量
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤

其他说明

更多信息，请见www.sigma-aldrich.com/genelutehp。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

靶器官

Central nervous system

储存分类代码

3 - Flammable liquids

闪点(°F)

77.0 °F - closed cup

闪点(°C)

25 °C - closed cup

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000393653
0000393655
0000380774
0000380774
0000393653

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Discovery of protein interactions using parallel analysis of translated ORFs (PLATO).
Larman HB
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Renal hypouricemia is a rare genetic disorder. Hypouricemia can present as renal stones or exercise-induced acute renal failure, but most cases are asymptomatic. Our previous study showed that two recessive variants of SLC22A12 (p.Trp258*, pArg90His) were identified in 90% of
Musaddeque Ahmed et al.
Nature communications, 12(1), 1781-1781 (2021-03-21)
Prostate cancer (PCa) risk-associated SNPs are enriched in noncoding cis-regulatory elements (rCREs), yet their modi operandi and clinical impact remain elusive. Here, we perform CRISPRi screens of 260 rCREs in PCa cell lines. We find that rCREs harboring high risk
Karina Yui Eto et al.
Antimicrobial agents and chemotherapy, 63(11) (2019-09-11)
Horizontal transfer of plasmids encoding antimicrobial resistance and virulence determinants has been instrumental in Staphylococcus aureus evolution, including the emergence of community-associated methicillin-resistant S. aureus (CA-MRSA). In the early 1990s, the first CA-MRSA strain isolated in Western Australia (WA), WA-5
Fraser Soares et al.
Blood, 137(16), 2171-2181 (2020-12-04)
Acute myeloid leukemia (AML) remains a devastating disease in need of new therapies to improve patient survival. Targeted adoptive T-cell therapies have achieved impressive clinical outcomes in some B-cell leukemias and lymphomas but not in AML. Double-negative T cells (DNTs)
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实验方案

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

Questions

1–6 of 6 Questions  

  1. Which format gives a better yield, vacuum or spin?

    1 answer

    1. There is no difference in plasmid recovery when using either format.

      Helpful?

  2. Do the binding columns fit on other manufacturers’ manifolds?

    1 answer

    1. Yes. The columns fit on all standard vacuum manifolds.

      Helpful?

  3. What is the expected yield of plasmid DNA that you can isolate from these kits?

    1 answer

    1. Yields can reach as high as 350 mg for a midiprep and 1.2 mg for a maxiprep. As with any protocol, lower yields can be expected with low copy plasmids.

      Helpful?

  4. How do the GenElute HP Plasmid Kits differ from other plasmid purification systems?

    1 answer

    1. The GenElute HP Plasmid Kits are much faster than other commercially available kits. A filter syringe is used for preparing the cleared lysate, which replaces a lengthy centrifugation step. These kits also offer the option of using a vacuum or spin purification format rather than a gravity drip to bind, wash and elute the plasmid. In addition, samples are eluted in Tris buffer so there are no alcohol precipitations required.

      Helpful?

  5. Is plasmid DNA isolated with this kit suitable for use in transfection?

    1 answer

    1. Yes. However, if you are currently required to use an endofree plasmid purification kit, we recommend using the GenElute HP Endotoxin-free Kit.

      Helpful?

  6. How long does it take to isolate plasmid using the vacuum format?

    1 answer

    1. A midiprep or maxiprep requires only 30 minutes to complete.

      Helpful?

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