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Merck
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主要文件

MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

别名:

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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About This Item

EC 号:
231-548-0
UNSPSC代码:
41116158
NACRES:
NA.54

用途

sufficient for 50 reactions

储存温度

20-25°C

相关类别

一般描述

印记®DNA修饰试剂盒包含完成亚硫酸氢盐转化和随后纯化DNA样本所需的所有试剂。DNA经化学变性，使亚硫酸氢盐试剂与单链DNA特异性反应，从而使胞嘧啶脱氨基并产生尿嘧啶残基。转化的DNA 适用于多种下游应用，包括甲基化特异性PCR、甲基化测序、焦磷酸测序以及甲基化微阵列。

应用

采用印迹®DNA修饰试剂盒进行DNA的亚硫酸氢修改。

特点和优势

  • 只需要50微微克的DNA或20个细胞
  • 整个流程用时不到2小时
  • 转化率大于99%
  • 降解率极低
  • 便捷一步协议选项
  • 一致且可重复的亚硫酸氢钠转换
  • 可用于基因组、内切核酸酶消化和FFPE DNA

储存及稳定性

所有组分均可在室温下储存。每瓶DNA修饰粉末足以进行10次DNA修饰。溶解后，溶液可在-20°C下避光保存一周。使用前，必须在室温下解冻冷冻溶液并涡旋2分钟。

法律信息

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural化学品安全说明书

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

补充剂危害

EUH031

储存分类代码

8B - Non-combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000333642
0000333642
SLCQ0447
SLCD4557
SLCD4559

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在文件库中查找您最近购买产品的文档。

访问文档库

The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates
Peat JR, et al.
F1000Research (2017)
Julian R Peat et al.
Methods in molecular biology (Clifton, N.J.), 1708, 161-169 (2017-12-11)
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA
Sarah Rose Huffman et al.
Methods in molecular biology (Clifton, N.J.), 925, 201-209 (2012-08-22)
Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
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Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

实验方案

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

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ChIP-qPCR Data Analysis

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ChIP-qPCR Data Analysis

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相关内容

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

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