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MDQ1

Sigma-Aldrich

Imprint® 甲基化 DNA 定量试剂盒

To measure global DNA methylation shifts from as low as 10 ng DNA

别名:

DNA Methylation Quantification, DNA methylation shift

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.54

一般說明

Imprint™ 甲基化 DNA 定量技术提供了一种快速可靠的方法来测量全局 DNA 甲基化移位。印记甲基化 DNA 定量试剂盒包含检测相对水平的甲基化 DNA 所需的所有试剂。最多 200 ng 纯化 DNA 与检测试剂条的微孔结合。使用捕获和检测抗体检测甲基化 DNA，然后用比色法定量。样本中存在的甲基化 DNA 的量与测量的吸光度成正比。

應用

Imprint® 甲基化 DNA 定量试剂盒已用于全局 DNA 甲基化分析。

特點和優勢

  • 基于 ELISA 的格式
  • 在 4 个易于遵循的步骤中完成程序
  • 提供的所有试剂，包括对照甲基化 DNA
  • 检测限为 5 ng 全甲基化 DNA
  • 输入 DNA 可能低至 10-200 ng
  • 程序在 3.5 小时内完成
  • 96 孔格式允许研究单样本或高通量研究
  • 对 5-mC 具有高特异性的优化抗体和试剂；对非甲基化胞嘧啶无交叉反应
  • 可与贴壁和悬浮细胞、组织、血浆及其他体液如唾液、尿液和血清一起使用

法律資訊

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • M8570Methylated Control DNA, 50 ng/μL化学品安全说明书

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象形圖

CorrosionExclamation mark
GHS05,GHS07

訊號詞

Warning

危險聲明

H290,H317,H412

危險分類

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Rafael Guerrero-Preston et al.
Epigenetics, 5(6), 539-546 (2010-06-05)
Environmental exposures in-utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero exposures to maternal smoking and perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation in umbilical cord serum. Our objective was
Magdalena Gryzinska et al.
Biochemical genetics, 51(7-8), 554-563 (2013-04-05)
DNA methylation is an epigenetic modification that plays an important role in the normal development and function of organisms. The level of DNA methylation is species-, tissue-, and organelle-specific, and the methylation pattern is determined during embryogenesis. DNA methylation has
Resistance training and redox homeostasis: Correlation with age-associated genomic changes
Dimauro I, et al.
Redox Biology, 10, 34-44 (2016)
Kazuhisa Nakano et al.
Journal of immunology (Baltimore, Md. : 1950), 190(3), 1297-1303 (2013-01-02)
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which fibroblast-like synoviocytes (FLS) exhibit an aggressive phenotype. Although the mechanisms responsible are not well defined, epigenetic determinants such as DNA methylation might contribute. DNA methyltransferases (DNMTs) are critical enzymes that
Alok Mishra et al.
Methods in molecular biology (Clifton, N.J.), 863, 15-31 (2012-02-24)
Epigenetics is an emerging science that can help to explain carcinogenesis. The possibility that carcinogenesis may originate in a stem cell process was proposed recently. Stem cells are generated and contribute to tumor formation during the process of tumor development.
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Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

Global DNA Methylation Quantification

Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.

实验方案

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

The Imprint® Methylated DNA Quantification Kit provides a high-throughput, non-radioactive means of measuring global DNA methylation shifts.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

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