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Merck

MAK144

Sigma-Aldrich

荧光测定细胞内活性氧试剂盒

sufficient for 200 fluorometric tests (orange)

别名:

荧光ROS检测试剂盒

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About This Item

EC 号:
UNSPSC代码:
12161503
NACRES:
NA.25
价格与库存信息目前不能提供

用途

sufficient for 200 fluorometric tests (orange)

应用

cosmetics
food and beverages

检测方法

fluorometric

相关疾病

cancer; neurological disorders

储存温度

−20°C

一般描述

在有氧呼吸期间氧气减少以及在细胞内各种酶体系的作用下,可生成活性氧类物质(ROS)。[1]在生理水平上,ROS有助于细胞信号传导和宿主防御能力。[2]当ROS生成增加至超过生物系统的解毒能力时,将导致氧化应激和细胞损伤。对细胞的主要损伤来源于ROS诱导的大分子改变,如膜脂质的多不饱和脂肪酸、必需蛋白质和DNA。[1][2]已有研究证明ROS与疾病状态有关,如阿尔茨海默病、帕金森病、癌症和衰老。[3]

应用

细胞内活性氧类物质(ROS)检测试剂盒(荧光法)已可用于评估机械通气(MV)对线粒体的损害。[4]它还被用于测量细胞内ROS的产生。[5]

适用性

本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。

原理

细胞内ROS检测试剂盒(荧光法)是一种灵敏的一步式荧光测定法,仅需1小时孵育即可检测活细胞中的细胞内ROS(特别是超氧化物和羟基自由基)。ROS与细胞可透过性传感器反应,产生的荧光产物(λex = 540/λem = 570 nm)与ROS含量成比例。该测定可使用方便的96孔或384孔微量滴定板。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Oxidized low density lipoprotein (ox-LDL) binding to ox-LDL receptor-1 in endothelial cells induces the activation of NF-KB through an increased production of intracellular reactive oxygen species.
Cominacini L, et al.
The Journal of Biological Chemistry, 275(17), 12633-12638 (2000)
High tidal volume induces mitochondria damage and releases mitochondrial DNA to aggravate the ventilator-induced lung injury.
Lin J, et al.
Frontiers in Immunology, 9, 1477-1477 (2018)
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants.
Das K and Roychoudhury A
Frontiers in environmental science., 2, 53-53 (2014)
Tao Zheng et al.
Frontiers in pharmacology, 12, 669146-669146 (2021-05-25)
Although miR-327 had a protective effect on cardiomyocytes as described previously, the potential mechanism still needs further exploration. The aim of this study was to investigate the role and mechanism of miR-327 on oxidative stress in myocardial ischemia/reperfusion injury (MI/RI)
Feng Gao et al.
Journal of cellular biochemistry, 121(11), 4450-4457 (2020-03-13)
The mechanisms driving the pathologic progression of osteoarthritis (OA) have not yet to be fully elucidated. Excessive and irreversible breakdown of the extracellular matrix is the main hallmark of OA. Inhibitors of DPP-4 have been widely used for over a

Questions

1–3 of 3 Questions  
  1. Does it work for non-cellular environments, like a solution? Thanks.

    1 answer
    1. This kit is not suitable for use in non-cellular environments and is intended only for the analysis of live cells as described in the Technical Bulletin.

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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